Protein Info for GFF5323 in Variovorax sp. SCN45

Annotation: Type IV pilus biogenesis protein PilQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 726 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF11741: AMIN" amino acids 48 to 130 (83 residues), 37 bits, see alignment E=4.6e-13 amino acids 160 to 262 (103 residues), 98.3 bits, see alignment E=3.8e-32 TIGR02515: type IV pilus secretin PilQ" amino acids 274 to 719 (446 residues), 503.9 bits, see alignment E=2e-155 PF03958: Secretin_N" amino acids 372 to 452 (81 residues), 51.4 bits, see alignment E=1.6e-17 PF00263: Secretin" amino acids 565 to 719 (155 residues), 157.6 bits, see alignment E=3.4e-50

Best Hits

Predicted SEED Role

"Type IV pilus biogenesis protein PilQ" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (726 amino acids)

>GFF5323 Type IV pilus biogenesis protein PilQ (Variovorax sp. SCN45)
MNQKKSTMAQWLRAAGLGLLTLSAWAVAHAQNAIESVTSSTQSGAEVIRIDLAQPLTAVP
AGFAVQTPARIALDFPGVTNAIGRSAIEVNQGNLRSVNVVQAGDRSRVVLNLKQATAYKA
EIQGKSLLVVLEPVAGAALAVSAATTFAENRNRDTLPLRDVDFRLGSDNTGRVIVDLPNN
QVGVDVKQQGKNLVVEFTKSTLPEGLRRRLDVADFGTPVQMITSQQSGDRVRMTIEAKGD
WEHSAYQSENQFVVEMRARKVDPTKLTQGAGYNGEKLSLNFQNIEIRSLLQVIADFTNFN
IVTSDSVTGALTLRLKDVPWDQALDIIMQAKNLGMRKNGSVLWIAPKDEINAKEKLEFEA
KATIENLEPLRTQSFQLNYTKAIAIAQGLTGTGASAGGGGGGGGGGTTSRILSPRGSVIA
ESRTNQLFVSDIPSRLAQIADLIQKLDIPVRQVLIEARIVEASDTFGKSLGVKLGGGVIN
RNNSFGTWPSVTGGGTTSTSTSTGSTTTQTSTTTSPTVTFGSPTSSNFINLPAGDAGGTG
NSAGSFAFSLFNSSFSRMLNLEISALEADGKGKLVSSPRVITADQTKALIEQGEEIPYQQ
ATSSGATSISFRKAVLKLEVTPQITPEGNIILTLDVSKDARGVNTSAGPAINTKHVQTEV
LVENGGTVVIGGIFELTETNDESRVPVLGEVPYLGALFRKRERVANKTEMLVFITPKMIT
DRNAAR