Protein Info for GFF526 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Heat shock protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 292 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 37 to 59 (23 residues), see Phobius details amino acids 162 to 183 (22 residues), see Phobius details amino acids 195 to 219 (25 residues), see Phobius details PF01435: Peptidase_M48" amino acids 79 to 288 (210 residues), 144.5 bits, see alignment E=1.7e-46

Best Hits

Swiss-Prot: 82% identical to HTPX_POLSJ: Protease HtpX homolog (htpX) from Polaromonas sp. (strain JS666 / ATCC BAA-500)

KEGG orthology group: K03799, heat shock protein HtpX [EC: 3.4.24.-] (inferred from 82% identity to pna:Pnap_3448)

Predicted SEED Role

"Heat shock protease"

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (292 amino acids)

>GFF526 Heat shock protease (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKRILLFVLTNFAVMAVLLITTRILGVDRFLTANGLNMTALAGFSLVIGFGGAIISLLIS
KPMAKFSAGVRVINQPQNADEAWIVETVRRFADKANIGMPEVGIFDGAPNAFATGAFKNS
ALVAVSTGLLQNMTKEEVEAVIGHEVAHVANGDMVTMTLIQGVMNTFVVFLSRVIGYAVD
AFLRRGSDNNSGPGIGYMVTTIVMDIVLGFLAAIIVAWFSRQREFRADAGAASLMGRKQP
MINALARLGGLTPGELPKTMQALGITGGLGKLFSTHPPIEERIAALQQQQQA