Protein Info for GFF5259 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Fumarylacetoacetase (EC 3.7.1.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 54% identity to pla:Plav_1652)Predicted SEED Role
"Fumarylacetoacetase (EC 3.7.1.2)" in subsystem Homogentisate pathway of aromatic compound degradation or Salicylate and gentisate catabolism (EC 3.7.1.2)
MetaCyc Pathways
- L-tyrosine degradation I (4/5 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.7.1.2
Use Curated BLAST to search for 3.7.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (335 amino acids)
>GFF5259 Fumarylacetoacetase (EC 3.7.1.2) (Hydrogenophaga sp. GW460-11-11-14-LB1) MKWQYTKGLHDIGNGNYAWLLPDGTWGWSNAGLVTDSGESLLVDTLFDLKLTREMLHAMR NAVPEAKEIAQVVNTHANGDHYFGNQLVADSEIIVSQACADEMLAHPPQALIDRFRNYKE LGDGGEFLMKAMGRVFDFRGIVYTPPTKTFSGEIDLKVGNKTVKLVEVGPAHTKGDVLAY IPEDKIVYTGDILFNGGHPIVWAGPVDNWIRACDLMLGWDVEVVVPGHGPITDKTGVRAL KHYLEYVKAEARKRYDQGMTLEQAVDDISLREFNSWTDAERIYVTVNNLYQEFSGDTSPP DTVKLFGLMAHYEERQRKLHGNIIGCGPNCNHANH