Protein Info for GFF5257 in Variovorax sp. SCN45

Annotation: ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 345 PF00005: ABC_tran" amino acids 52 to 205 (154 residues), 123.1 bits, see alignment E=1.4e-39 TIGR01727: oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain" amino acids 255 to 338 (84 residues), 92.7 bits, see alignment E=5.9e-31 PF08352: oligo_HPY" amino acids 256 to 320 (65 residues), 75.4 bits, see alignment E=3.5e-25

Best Hits

Swiss-Prot: 52% identical to APPF_BACSU: Oligopeptide transport ATP-binding protein AppF (appF) from Bacillus subtilis (strain 168)

KEGG orthology group: K02032, peptide/nickel transport system ATP-binding protein (inferred from 96% identity to vpe:Varpa_1177)

MetaCyc: 52% identical to dipeptide ABC transporter ATP binding subunit DppF (Escherichia coli K-12 substr. MG1655)
ABC-8-RXN [EC: 7.4.2.9]

Predicted SEED Role

"Putative glutathione transporter, ATP-binding component" in subsystem Utilization of glutathione as a sulphur source

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (345 amino acids)

>GFF5257 ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) (Variovorax sp. SCN45)
MSAVIEPRGNAPAAAGEPLVVAHDLARTFDVSPPWLNRVLERKPRVLLHAVDGVSFSIER
GKTLALVGESGCGKSTVARLLVGLYAPTRGGLQFDGQDAHAAFKTSEGRKLRRRIQMIFQ
DPYASLNPRWIVEDIIGEPLREHGIISDKAALRERVGELLKSVGLSPLDMSKYPHQFSGG
QRQRISIARALATQPEFLVCDEPTSALDVSVQAQVLNIMKDLQREQGLTYLFISHNLAVV
RHVADQVGVMYLGRLVEVADKQKLFAAPQHPYTRMLLDAIPQMKHTGRSRTPVQGEVPNP
LNPPTGCAFHPRCPHANARCSAERPKLLGIAGVQVACHAVEEGRI