Protein Info for GFF5255 in Pseudomonas sp. DMC3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 794 transmembrane" amino acids 20 to 43 (24 residues), see Phobius details amino acids 311 to 333 (23 residues), see Phobius details PF03924: CHASE" amino acids 88 to 269 (182 residues), 121.9 bits, see alignment E=1e-38 PF13188: PAS_8" amino acids 364 to 417 (54 residues), 19.3 bits, see alignment 3e-07 PF00989: PAS" amino acids 364 to 490 (127 residues), 43 bits, see alignment E=1.5e-14 TIGR00229: PAS domain S-box protein" amino acids 372 to 499 (128 residues), 52.6 bits, see alignment E=4.8e-18 PF13426: PAS_9" amino acids 375 to 492 (118 residues), 40 bits, see alignment E=1.5e-13 PF08447: PAS_3" amino acids 531 to 605 (75 residues), 41.5 bits, see alignment E=4.7e-14 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 632 to 790 (159 residues), 152.4 bits, see alignment E=9.5e-49 PF00990: GGDEF" amino acids 636 to 788 (153 residues), 143.7 bits, see alignment E=1.6e-45

Best Hits

KEGG orthology group: None (inferred from 89% identity to pfo:Pfl01_1789)

Predicted SEED Role

"CHASE domain/PAS domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (794 amino acids)

>GFF5255 hypothetical protein (Pseudomonas sp. DMC3)
MPLHPVRPKILGFISEDVSAWLVALLVLLAGGILTGLLAWGTFNQFQQQSRQRFQLLANE
RYSRIAERFQDQEQRLDGLRRFFANSESVSRAEFDGYSRPLLLRTQAYTYAPRVSGAERA
AFEQQVRDEGLSEFTIRELDAKGELQLAAPREEYVPVLYTQTQSRLAPPLGYDLLAQPLR
RETLQRADQLGSMAVSQPMHLVGIEPSYARGVLLLAPVSRAGESQPFGYVMAVISMRQLL
ADGLPDALHDYLSVRILDLSTQDQHEALFESSNEAVPGDLAATRLLRMADHDYQVDILPS
EAFMQANHSSVGSVIILGGLLSALLSALLYVLVSQRQRAVRLVEVRTQELHEREQELRGT
HGQLRGVLNAATQVAIIATDLRGVINTFNPGAEQMLGYSSAEVVGRMTLENLHLPRELAA
RSAELSARYGKSIPTCHAMLVEGGEVGGHEAREWTLVRSDGSHLPVNMLATPVLDEQGLW
VGHLAICIDITERKRVHEVLAARDTLLKKLSAHVPGGIYQFKMEFDGRFSVIYASDGIRE
IYELEPDVLLLDSEAIFSRIHPQDAIRVRSSIRESAENLSPWREEYRVQLPERGLRWVRG
EATPEELPGGGVLWHGYISDISDLKRVEEELRALSITDSLTGIHNRRYFQERLTTEMARV
ERGGGELSVIMLDIDHFKRINDQFGHAVGDRVLQAVCERIGHRLRRTDVFCRLGGEEFMV
LCPDIDGEHAYMLAMELWEGLRSAPVDVVGLVTASFGIASWRPGEGADALLLRADSGVYA
AKQNGRDRVEREMS