Protein Info for GFF5249 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Ribonuclease BN (EC 3.1.-.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 transmembrane" amino acids 44 to 71 (28 residues), see Phobius details amino acids 112 to 131 (20 residues), see Phobius details amino acids 152 to 176 (25 residues), see Phobius details amino acids 192 to 212 (21 residues), see Phobius details amino acids 223 to 246 (24 residues), see Phobius details amino acids 265 to 285 (21 residues), see Phobius details TIGR00765: YihY family inner membrane protein" amino acids 32 to 285 (254 residues), 214 bits, see alignment E=1.7e-67 PF03631: Virul_fac_BrkB" amino acids 39 to 285 (247 residues), 191.1 bits, see alignment E=1.4e-60

Best Hits

Swiss-Prot: 62% identical to Y2991_RHOFT: UPF0761 membrane protein Rfer_2991 (Rfer_2991) from Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)

KEGG orthology group: K07058, membrane protein (inferred from 62% identity to pol:Bpro_1830)

Predicted SEED Role

"Ribonuclease BN (EC 3.1.-.-)" in subsystem LMPTP YfkJ cluster or tRNA processing (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (429 amino acids)

>GFF5249 Ribonuclease BN (EC 3.1.-.-) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTLKERLRALETLARALWRDALNFPWRNTALTLRERFREDRLGVTASSLTFTTTISLVPL
FTVALAIFSAFPMFDRLQLTLQRWLVQSLVPPDIAKQVFSYLNQFANKAGQMGWAGALVL
LVTALALILTIDRKLNDIWRVRQTRSLTQRVLVYWAVLTLGPLLLAGSLAATSYAVTASR
GVVSGLPGGVRFLLDASQFVLLTGGLAALYRYVPNTRVRWSHALSGAVFVGVGLELAKKV
LAWYLAQVPTYSVVYGTFATVPILLVWFYVAWVIVLLGAVVAAYLPSLLSGIARRSDTPG
WSFQLALEVLAQLQLARDTVPKGLSLEALAQVLRVDALQLEEPVAVMVKLDWLGRLDEDG
ERYVLLTDLAMTPVAPLVERLLLAHQPHTARFWSESRWMDLSVAQAVSATPALPGGARET
VGEPSVASG