Protein Info for GFF5248 in Pseudomonas sp. DMC3

Annotation: Serine/threonine-protein kinase PknD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 transmembrane" amino acids 440 to 457 (18 residues), see Phobius details amino acids 534 to 554 (21 residues), see Phobius details PF13672: PP2C_2" amino acids 17 to 196 (180 residues), 38.8 bits, see alignment E=1.6e-13 PF00069: Pkinase" amino acids 262 to 460 (199 residues), 113.8 bits, see alignment E=1.9e-36 PF07714: PK_Tyr_Ser-Thr" amino acids 284 to 502 (219 residues), 57 bits, see alignment E=3.8e-19

Best Hits

KEGG orthology group: None (inferred from 88% identity to pfo:Pfl01_1782)

Predicted SEED Role

"Serine/threonine protein kinase (EC 2.7.11.1)" (EC 2.7.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.11.1

Use Curated BLAST to search for 2.7.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (556 amino acids)

>GFF5248 Serine/threonine-protein kinase PknD (Pseudomonas sp. DMC3)
MALQLSFAEASATGPRAENQDALRSVTPAPALSVSKGYLFAIADGVSQCADGGLAARSTL
QALALDYYATPETWSVAQALDRLLLAQNRWLQANGGGQPLLTTVSALVMRGRRFTLAHVG
DCRVYRWHAERLQRISEDHVWEQPGMQHVLKRALGLDQHLVMDFLDGELREGESFVLLSD
GVWSTLGDTAIAAILRDQVDLPSAAQTLVNAAHMAGSQDNASALLVRVDTLGEASIGDAL
VQLQQWPLPPTLKPGQAFEGWQVQGIIGQSQQSLLYRVLDSQGQPWLLKTLPTRLADDPQ
AGQALLSEEWFLKRVAGRHFPQIHACTQRQHLYYVMREYAGTTLADVFQHSGPLPLAQWQ
DIAERLLRAVGLLHRRQILHRDIKPENLLQGDDGELRLLDFGLAYCPGLSEDAPSTLPGT
PSFIAPEAFRGESPSAQQDLYSVGVTLYFLLTGHFPYGEIEAFQRPRFGVPVSASRYRPD
LPEWLAQSLERGVAADPQQRFETAEEWLLVLEQGERQSLSVRPRPLLEREPLKVWRTMAL
LALLGNVVLLVLLFHS