Protein Info for GFF5246 in Variovorax sp. SCN45

Annotation: Threonine dehydratase biosynthetic (EC 4.3.1.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 526 TIGR01124: threonine ammonia-lyase, biosynthetic" amino acids 14 to 524 (511 residues), 797.7 bits, see alignment E=2e-244 PF00291: PALP" amino acids 32 to 316 (285 residues), 254.5 bits, see alignment E=1.4e-79 PF00585: Thr_dehydrat_C" amino acids 329 to 430 (102 residues), 63.4 bits, see alignment E=1.5e-21 amino acids 442 to 524 (83 residues), 84.7 bits, see alignment E=3.3e-28

Best Hits

Swiss-Prot: 52% identical to ILVA_PASMU: L-threonine dehydratase biosynthetic IlvA (ilvA) from Pasteurella multocida (strain Pm70)

KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 93% identity to vpe:Varpa_1166)

Predicted SEED Role

"Threonine dehydratase biosynthetic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 4.3.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.19

Use Curated BLAST to search for 4.3.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (526 amino acids)

>GFF5246 Threonine dehydratase biosynthetic (EC 4.3.1.19) (Variovorax sp. SCN45)
VRNAPVTPALTPADYLRKILNARVYDVAVESALEKARALSERLGNTVLLKREDQQPVFSF
KLRGAYNKMAHLSAAQLASGVICASAGNHAQGVALGARKLGTRAVIVMPVTTPKLKIDAV
RGFGGEIVLHGDSYSDAYLHALELQAQQNLTFVHPFDDPDVIAGQGTIAMEILRQHQGPL
DAVFVAIGGGGLISGVANYIKAVRPEIKVIGVQMNDSDAMMQSVAAHQRVNLPDVGLFSD
GTAVKLVGEETFRIANELVDDYIAVDTDAVCAAIKDIFVDTRSIVEPAGALAVAAIKEYA
ARHGRQGETYAAILCGANMNFDRLRFVAERAEVGEEREALFAVTIPEERGSFRRFCELIS
EIPASDAEGGGAPRNVTEFNYRISDAAKAHVFVGLTTSARGESATIARTFNDHGFDSLDL
THDELAKEHLRHLVGGRTGLAHDERLLRFVFPERPGALLKFLSLMRPNWNISLFHYRNQG
ADYGRILVGLQVPAGESTEFAAFLETLGYPYVEETANPAYRLFLQA