Protein Info for GFF5245 in Pseudomonas sp. DMC3

Annotation: Nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 903 PF04879: Molybdop_Fe4S4" amino acids 4 to 52 (49 residues), 61.3 bits, see alignment 1.4e-20 PF00384: Molybdopterin" amino acids 63 to 488 (426 residues), 239.8 bits, see alignment E=9.4e-75 PF01568: Molydop_binding" amino acids 587 to 695 (109 residues), 86.4 bits, see alignment E=2.8e-28 PF04324: Fer2_BFD" amino acids 848 to 894 (47 residues), 31.4 bits, see alignment 3.9e-11

Best Hits

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 92% identity to pfo:Pfl01_1779)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (903 amino acids)

>GFF5245 Nitrate reductase (Pseudomonas sp. DMC3)
MNRQTTASTCCYCGVGCGVLIEHDGERILGVSGDPAHPANFGKLCSKGSTLHLTGDLAAR
ALYPELRLGKGLARSRTDWDTALDHATNVFAETIAEHGPDSVAFYISGQLLTEDYYAFNK
LARALVGTNNIDSNSRLCMSSAVVGYKRSLGADAPPCSYEDLELSDCVMIVGSNMAYAHP
VLFRRLEEAKSRRPQMKVIVIDPRRTDTCDLADLHLAILPGTDVALFHGILHLLLWEDWI
DRDFIKAHTEGLAELKTLVRDYTPQMVSQLCGISIEQLQQCAEWVGTAPSFLSLWCMGLN
QSSAGSAKNSALINLHLATGQIGRPGAGPFSLTGQPNAMGGRETGSLSNLLPGHRDAANP
EHRAEVAAYWGVESLPEAPGLSAIELFENLRSGKIKALWIACTNPAQSMPDQSAVRAALE
ACPFVVLQEAFRTTETAAFADLLLPAASWGEKEGSVTNSERRISHVRKAVVAPGEARPDW
AITVDFAQRLEKRLRPKQPSLFAFDQPSQLFDEFKALTRGRDLDLSGISHALIDEIGPQQ
WPFPAGARQGTPRLYGDGIFPTANGRAQFIADPYRAAKEQRDARFPLTLITGRLRDQWHG
MSRTGTAAQLFGHVSEAVLSLHPDEMRRHRLQPGDLVSLKSRRGAVIVAVGSDDSVRPGQ
AFLPMHWGDRYLKGGVNTLTLPALDPLSKQPELKHSGVRLEAVNLPWQLFALIEGDVQRH
FETLRPLCEAFSYVSLSLVGRERPALLIRAAHSQAPDPQLLGEIDQCLSLLDGPVLAYDD
PRRAIGKRVRIENGRITAIRLAGETLAQHWLQSLWLEGRADQQLRRWLLAPMSAPPGAVG
AIASDKTLCNCENVSLNAVCAGIRQGLDLQGLKNNLRCGTQCGSCVPEIKRLLAAEARPV
AVI