Protein Info for GFF5232 in Sphingobium sp. HT1-2

Annotation: Inner membrane transport permease YhhJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 signal peptide" amino acids 22 to 22 (1 residues), see Phobius details amino acids 42 to 42 (1 residues), see Phobius details transmembrane" amino acids 23 to 41 (19 residues), see Phobius details amino acids 175 to 200 (26 residues), see Phobius details amino acids 221 to 245 (25 residues), see Phobius details amino acids 257 to 278 (22 residues), see Phobius details amino acids 288 to 308 (21 residues), see Phobius details amino acids 315 to 337 (23 residues), see Phobius details amino acids 345 to 367 (23 residues), see Phobius details PF12679: ABC2_membrane_2" amino acids 13 to 368 (356 residues), 48.6 bits, see alignment E=1.4e-16 PF12698: ABC2_membrane_3" amino acids 24 to 364 (341 residues), 141.1 bits, see alignment E=9.9e-45 PF12730: ABC2_membrane_4" amino acids 166 to 276 (111 residues), 29.3 bits, see alignment E=1.6e-10 PF01061: ABC2_membrane" amino acids 169 to 337 (169 residues), 80.9 bits, see alignment E=2.1e-26

Best Hits

KEGG orthology group: K09686, antibiotic transport system permease protein (inferred from 66% identity to bmj:BMULJ_05773)

Predicted SEED Role

"ABC-type multidrug transport system, permease component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (375 amino acids)

>GFF5232 Inner membrane transport permease YhhJ (Sphingobium sp. HT1-2)
MTRWILNVALLSGKELRSVMKDVTLMALILFAFTVAIQLVANGVRAEVMNASVAIMDEDH
SELSRRLRDAIQQPYFKPPEDIERSAIGPAMNSGKYIFVIEIPPRFEADTLAGRSPSVQI
LVDATAMTQAGLGAAYFQQIFAQETLDFLHARGLLEALPVQVESRMHFNPNAQSAWYTSV
MQIVTNVTVLSIILVGAAVIREREHSTIEHLLVMPVRASEIAFAKILANGLVILLASIAS
LWFVVHGVLGVPLTGSLLLFTAAMFLYLFSVTSLGMWMATLAPSMPQFGLFAVPVYAIAY
LLSGAATPLESMPSAIRTIALCLPTTQFVSLSQAILYRGAGVRTILPELAIICGSGVFFV
AMAVSRFRSMLSRQG