Protein Info for PS417_26720 in Pseudomonas simiae WCS417

Annotation: twitching motility protein PilT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 344 TIGR01420: twitching motility protein" amino acids 3 to 342 (340 residues), 475 bits, see alignment E=7.5e-147 PF00437: T2SSE" amino acids 11 to 271 (261 residues), 152.5 bits, see alignment E=6.3e-49

Best Hits

Swiss-Prot: 74% identical to PILT_PSEAE: Twitching mobility protein (pilT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02669, twitching motility protein PilT (inferred from 94% identity to pfs:PFLU5762)

Predicted SEED Role

"Twitching motility protein PilT" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1UFP2 at UniProt or InterPro

Protein Sequence (344 amino acids)

>PS417_26720 twitching motility protein PilT (Pseudomonas simiae WCS417)
MDITELLTASVHRGASDLHLSAGLAPMLRVDGEVWPLEGPVLSPPQVADLLSPLLNQHQQ
KDFETSLETDFAFELPDVARFRANVFRQDRGLSAVFRTIPSEVRSLESLGLGEVFQRIAQ
LPRGLVLVTGPTGSGKSTTLAAMIDFLNQHRRHHILTLEDPIEFIHTPKMALINQRQVHR
DTHGFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQ
MVSAIQTGGALGMKTLDMSLKVLVGEGLVSREDAREKARVPTDI