Protein Info for GFF5218 in Variovorax sp. SCN45

Annotation: ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 PF00005: ABC_tran" amino acids 22 to 164 (143 residues), 122.9 bits, see alignment E=3.9e-39 PF17912: OB_MalK" amino acids 240 to 282 (43 residues), 27.3 bits, see alignment 1.4e-09 PF08402: TOBE_2" amino acids 275 to 357 (83 residues), 25.2 bits, see alignment E=3.6e-09

Best Hits

Swiss-Prot: 45% identical to UGPC_RUEPO: sn-glycerol-3-phosphate import ATP-binding protein UgpC (ugpC) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K02010, iron(III) transport system ATP-binding protein [EC: 3.6.3.30] (inferred from 98% identity to vpe:Varpa_1138)

Predicted SEED Role

"Ferric iron ABC transporter, ATP-binding protein" in subsystem Iron acquisition in Vibrio or Transport of Iron

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.30

Use Curated BLAST to search for 3.6.3.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (359 amino acids)

>GFF5218 ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines) (Variovorax sp. SCN45)
MTSSSFLSLRGIGKSFGATRVLDGIDLDIEPGEFVCLLGPSGCGKTTLLRIVCGIERADS
GQILLGGQDIAGLPPAARGFGVVFQSYALFPNLTAAQNIAYGLHPTGALAREMAKRSREM
LDLVGLAAHADKYPVQLSGGQQQRVALARALAPNPRLLLLDEPLSALDAQVRASLRSEIR
ALQKRLGIVTIMVTHDQEEALSMADRVVLMHNGRIEQAGTPDELYRQPRTRFVAGFVGRM
NMLPATVLADGKVRVRDSELFCAPGNLSVGSQATVGIRPEHVVVKRYGTELGANSFAARL
LDTEFFGNRTSARLACEPLGIEIEAELPADARGGGDEPLVPSSMVHIQLPLNALSVLAH