Protein Info for GFF521 in Variovorax sp. SCN45

Annotation: Secretion protein HlyD precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 378 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 47 to 367 (321 residues), 223.7 bits, see alignment E=1.5e-70 PF16576: HlyD_D23" amino acids 60 to 287 (228 residues), 64.5 bits, see alignment E=1.8e-21 PF13533: Biotin_lipoyl_2" amino acids 79 to 120 (42 residues), 25.5 bits, see alignment 1.7e-09 PF13437: HlyD_3" amino acids 184 to 283 (100 residues), 64.7 bits, see alignment E=2.4e-21

Best Hits

KEGG orthology group: None (inferred from 85% identity to vap:Vapar_2411)

Predicted SEED Role

"Secretion protein HlyD precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (378 amino acids)

>GFF521 Secretion protein HlyD precursor (Variovorax sp. SCN45)
MKTMKRSTLVTLLIVLAVVIAAVVFMTRKPADKAAAAGPAPKPALTVTVAAPEATELTLT
LAANGNVAAWQEAVVGSESNGLKLAEVRVNVGDTVKKGQVLAVFSPETVRADIAQSRASL
AEARATAADAAGNAARARTLQATGALSQQQINQYQTTEQTAKARVEAAEAVLAAQEVRGR
NTQVLAPDDGVISSRTATVGSVVAAGTELFKLIRQGRLEWRAEVTSAELSRIAVGTNAFV
VSASGAQVRGKVRSIAPTVDPQTRAALVYVDLPNVQQNTGIKAGMFARGDFELGRSTAPT
VPQSAIVPRDGFNNVFLLKPDNHVAQLKVQVGRRLNDRIEITSALPQGAKVVVQGAGFLN
DGDLVRVVDTPPTAAAAK