Protein Info for GFF5207 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 PF23441: SDR" amino acids 7 to 216 (210 residues), 27.2 bits, see alignment E=6.2e-10 PF00106: adh_short" amino acids 10 to 212 (203 residues), 189 bits, see alignment E=1.6e-59 PF08659: KR" amino acids 12 to 123 (112 residues), 55.9 bits, see alignment E=1.4e-18 PF01370: Epimerase" amino acids 12 to 193 (182 residues), 26.5 bits, see alignment E=1e-09 PF13561: adh_short_C2" amino acids 16 to 258 (243 residues), 190.8 bits, see alignment E=7.2e-60

Best Hits

KEGG orthology group: K00540, [EC: 1.-.-.-] (inferred from 83% identity to pol:Bpro_1896)

Predicted SEED Role

"3-hydroxybutyrate dehydrogenase (EC 1.1.1.30)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 1.1.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-, 1.1.1.30

Use Curated BLAST to search for 1.-.-.- or 1.1.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (260 amino acids)

>GFF5207 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MAYSLDLSGRVALITGASGGLGEQFAKTLARAGAAVVLASRRTDRLMALRAHIEAAGGDA
HVVAMDVTDTASIKAAVAHAETEVGAIDILVNNSGVSTTQRLQDVTEEDYDFVFDTNTKG
AFFVAQEVGRRMLARANGAAPGTYVGGRIVNIASMAGLKVLPQIGVYCMSKAAVVQMTKA
MAIEWGRFGINVNAICPGYIDTELNHHHWQTEQGQKLINMLPRKRIGSPADLDALLVMLC
SSESHFVNGAVIAADDGFGA