Protein Info for GFF5172 in Variovorax sp. SCN45

Annotation: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 626 (625 residues), 782.8 bits, E=1.2e-239
• PF13522: GATase_6
  • amino acids 63 to 188 (126 residues), 61.1 bits, E=1.8e-20
• PF13537: GATase_7
  • amino acids 101 to 207 (107 residues), 55.1 bits, E=1.2e-18
• PF01380: SIS
  • amino acids 308 to 431 (124 residues), 93.3 bits, E=1.6e-30
  • amino acids 479 to 606 (128 residues), 81.8 bits, E=5.9e-27

Best Hits

Swiss-Prot: 73% identical to GLMS_BORPA: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 96% identity to vpe:Varpa_1068)

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (5/24 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.16

Protein Sequence (626 amino acids)

>GFF5172 Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16) (Variovorax sp. SCN45)
MCGIVGAASHRNIVPVLVQGLQRLEYRGYDSCGVAVHAGGLTRARTTSRVADLVAQVRDD
KVEGLTGIAHTRWATHGAPAVHNAHPHFSHGPGADAQAARPGRIALVHNGIIENHETLRA
ALEAKGYVFESQTDTEVIAHLVDSLYDGDLFEAVKAAVLQLHGAYAIAVICRDEPQRVVG
ARAGSPLILGAGKDGTENFLASDAMALAGVTDQIVYLEEGDVVDVQPGKYWIVDKNHKPV
ARQVRTVHAHSGAAELGPYRHYMQKEIFEQPRAIGDTLEGVEGIVPELFDGIGQDGATGA
SAHRVFQDIDKILILACGTSYYSGCTAKYWLESIAKISTQVEIASEYRYRDSVPDPKTLV
VTISQSGETADTLAALKHARSLGMEQTLTICNVATSAMVRECKLAYITRAGVEIGVASTK
AFTTQLAGLFLLTLAIAQSKGRLSEADEARYLKEMRHLPVALQSVLALEPQIIGWAEDFA
RKENALFLGRGLHYPIALEGALKLKEVTYIHAEAYAAGELKHGPLALVTNEMPVVAVAPN
DALLEKLKSNLQEVRARGGVLYVLADGDTKITSSEGLHVIRMPEHYGALSPILHVVPLQL
LAYHTAGARGTDVDKPRNLAKSVTVE