Protein Info for GFF5170 in Variovorax sp. SCN45

Annotation: N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 14 to 472 (459 residues), 580.6 bits, E=1.1e-178
• PF12804: NTP_transf_3
  • amino acids 16 to 165 (150 residues), 67.6 bits, E=2.3e-22
• PF00483: NTP_transferase
  • amino acids 16 to 209 (194 residues), 42.9 bits, E=7.1e-15
• PF00132: Hexapep
  • amino acids 334 to 369 (36 residues), 28.7 bits, 1.1e-10
  • amino acids 412 to 446 (35 residues), 29.9 bits, 4.9e-11

Best Hits

Swiss-Prot: 75% identical to GLMU_POLSJ: Bifunctional protein GlmU (glmU) from Polaromonas sp. (strain JS666 / ATCC BAA-500)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 90% identity to vpe:Varpa_1066)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• peptidoglycan recycling I (9/14 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (5/24 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (475 amino acids)

>GFF5170 N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157) (Variovorax sp. SCN45)
MNQTPQQDTPGPVDIVIMAAGKGTRMKSRLPKVLHRLAGRALLAHVAGTAGRIGARDVVV
VTGHGAAEVEAAMAGGIAGATLRFARQEPQLGTGHAVLQAAPLLPDDGTVVVLSGDVPLI
GEDTLRALVAASGGQRLALLTIEFDDPTGYGRVIRSAAAGDVTAVVEHKDATEAQRAVRE
IYSGVMAVPARLLKGWLARLDNRNAQGEYYLTDVVKFAAADGVPVVAHVTTDALQVAGIN
SPAQLAALERAWQLRQANALMEQGVRLADPARFDLRGTLECAADVEIDVNCVFEGAVSLG
EGVRIGANCVIANARIEAGAVIHPFTHIEGEKAGVTVGAGALIGPFARLRPGAQLGAEVH
IGNFVEVKNSTLAAGAKANHLAYLGDATVGERVNYGAGSITANYDGANKHRTVIGDDVHV
GSNCVLVAPVTIGAGGTIGGGSTVNKSTEPGALTVSRAKQVSFPNWKRPQKKPKA