Protein Info for PS417_26360 in Pseudomonas simiae WCS417

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 282 PF00561: Abhydrolase_1" amino acids 30 to 254 (225 residues), 50.4 bits, see alignment E=2.5e-17 PF12697: Abhydrolase_6" amino acids 31 to 264 (234 residues), 42.1 bits, see alignment E=1.7e-14

Best Hits

Swiss-Prot: 65% identical to RHLA_PSEAE: 3-(3-hydroxydecanoyloxy)decanoate synthase (rhlA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 94% identity to pfs:PFLU5689)

MetaCyc: 65% identical to 3-(3-hydroxyalkanoyloxy)alkanoate synthase (Pseudomonas aeruginosa PA14)
RXN-11762

Predicted SEED Role

"RhlA, 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs) synthase" in subsystem Rhamnolipids in Pseudomonas

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UT26 at UniProt or InterPro

Protein Sequence (282 amino acids)

>PS417_26360 MFS transporter (Pseudomonas simiae WCS417)
MNHGSFVIEKLFKHYNVHVEQLGNDSAKKTVLMVNGALSTTRSFARTSKCLAEHFNVLLF
DLPFSGYSREHNTDLDLVTKDDEVHMLRALVERFQVNHLVSASWGGISTLLTLAHNPPSI
ESSVVMALAPNLNQAMLDYVERVRVLIEADDKSAVGHLLNETVGKYLSPRLKRNNHRHLS
TMATTEYRQARFHIHQVLALGDGNYLPALRQIETPVHFLNGALDEYTPAAEAQLFKQYVG
RSSFAVAEQTGHLLDLESNEAAVAVHRELLDFLVGKHATVGA