Protein Info for GFF5136 in Pseudomonas sp. DMC3

Annotation: Isopenicillin N epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00266: Aminotran_5
  • amino acids 58 to 368 (311 residues), 106.8 bits, E=1.9e-34
• PF00155: Aminotran_1_2
  • amino acids 64 to 209 (146 residues), 39.6 bits, E=5.7e-14
• PF01041: DegT_DnrJ_EryC1
  • amino acids 72 to 209 (138 residues), 28.8 bits, E=1.1e-10

Best Hits

KEGG orthology group: None (inferred from 88% identity to pfo:Pfl01_4359)

Predicted SEED Role

"Isopenicillin N epimerase (EC 5.1.1.17)" (EC 5.1.1.17)

MetaCyc Pathways

• deacetylcephalosporin C biosynthesis (1/3 steps found)
• superpathway of penicillin, cephalosporin and cephamycin biosynthesis (1/10 steps found)

KEGG Metabolic Maps

• Penicillin and cephalosporin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.1.17

Protein Sequence (393 amino acids)

>GFF5136 Isopenicillin N epimerase (Pseudomonas sp. DMC3)
MPDNTRRARDEAFWQTFADRYDVQPGPVNLENGYFGRMSRTVIEEYQRNIELINTSNSIY
ARQRFEQHDNLEIRAQLAELIGVRAQSVALTRNATEGLQSLIRNYNRLQPGDQVLICDLE
YDTVKGAMRWLAQQRGVEVIEITHQHPASFDSLLESYREAFTRHPKLKLMALTHVTHRTG
LVMPVQAIAALAKEHGVDIILDGAHALGQIEFDLQALGIAFAGYNLHKWIGAPLTLGFIY
IAPQRLADIDPDMGEMHYPITDIRARTSYSTPNIPALMTLPLVFEEHDALGGAAAKSPRL
NYLRNLWVSAVRHLPGIEVLTPDDPQLYCGITALRFTRHADQQAMAERLLSEFNLFTVVR
TGAACGPCIRITPGLTTTAGEMEQLVHALNQLR