Protein Info for GFF5136 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Aspartokinase (EC 2.7.2.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00656: aspartate kinase, monofunctional class
  • amino acids 1 to 417 (417 residues), 411.4 bits, E=4.3e-127
• PF00696: AA_kinase
  • amino acids 3 to 234 (232 residues), 177.5 bits, E=7.6e-56
• TIGR00657: aspartate kinase
  • amino acids 67 to 415 (349 residues), 366.6 bits, E=2.2e-113
• PF22468: ACT_9
  • amino acids 278 to 337 (60 residues), 32.3 bits, E=1.3e-11
  • amino acids 357 to 415 (59 residues), 87.4 bits, E=8.5e-29
• PF01842: ACT
  • amino acids 281 to 337 (57 residues), 31.5 bits, 2.4e-11
• PF13840: ACT_7
  • amino acids 352 to 413 (62 residues), 57.2 bits, E=2.5e-19

Best Hits

KEGG orthology group: K00928, aspartate kinase [EC: 2.7.2.4] (inferred from 89% identity to aaa:Acav_2806)

MetaCyc: 59% identical to aspartokinase (Halomonas elongata DSM 2581)
Aspartate kinase. [EC: 2.7.2.4]

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis (EC 2.7.2.4)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• L-lysine biosynthesis II (7/9 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• spermidine biosynthesis II (2/4 steps found)
• ectoine biosynthesis (2/5 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (3/7 steps found)
• norspermidine biosynthesis (2/6 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• superpathway of polyamine biosynthesis III (2/8 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.4

Protein Sequence (426 amino acids)

>GFF5136 Aspartokinase (EC 2.7.2.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MALIVHKYGGTSMGSTERIRNVARRVAKWHRAGHQLVVVPSAMSGETNRLLGLAKELAPG
KTDSAYNRELDMLAATGEQASSALLAIALQAEGIGSVSYAGWQVPIKTNSAFTKARIESI
DDVRIRADLAASKVVIVTGFQGVDPEGHITTLGRGGSDTSAVAVAAALKAAECLIYTDVD
GVYTTDPRVVPEARRLQRVSFEEMLEMASMGSKVLQIRSVEFAGKYKVPLRVLSSFTPWD
IDLNEEAASGTLITFEEDEDMEQAVVSGIAFNRDEAKISVLGVPDTPGIAYQILGAVADA
NIEVDVIIQNLSKDGKTDFSFTVHRNDFQKTMDLLKAKVVPTLGAAEVVGDTRICKVSIV
GIGMRSHVGVASRMFKCLSVEGINIQMISTSEIKTSVVIDEKYMELAVRSLHREFDLDQT
ADTITA