Protein Info for GFF5131 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Tricarboxylate transport sensor protein TctE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 469 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details transmembrane" amino acids 17 to 24 (8 residues), see Phobius details amino acids 170 to 188 (19 residues), see Phobius details PF08521: 2CSK_N" amino acids 14 to 165 (152 residues), 136.1 bits, see alignment E=1.4e-43 PF00512: HisKA" amino acids 243 to 304 (62 residues), 45.7 bits, see alignment E=8.2e-16 PF02518: HATPase_c" amino acids 353 to 452 (100 residues), 71.3 bits, see alignment E=1.4e-23

Best Hits

KEGG orthology group: K07649, two-component system, OmpR family, sensor histidine kinase TctE [EC: 2.7.13.3] (inferred from 64% identity to pna:Pnap_1232)

Predicted SEED Role

"Tricarboxylate transport sensor protein TctE"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (469 amino acids)

>GFF5131 Tricarboxylate transport sensor protein TctE (Hydrogenophaga sp. GW460-11-11-14-LB1)
LRRTLLMGILLPVFAFVVINTVVLYRQALDAADTAYDRTLLATAKSLGEQLRVTGSGENV
RVESTLLYSALEAFEADNRSRIFYRVNGLKGEMVSGFDDMPDWRRDIPQKNLYAALVNFY
DDEYRGMPVRMAVLLQPVSGVYEQGMATIQVAETLELRQTLARQILIDTLWRQAGLVVVI
ALVVVFVVQRATRPVRALSHALGMRNENDLSPLSTQDAPRELLPLLDATNQHMQRLSHLL
EHQKRFVRDTSHQLRTPLAVLKTQVQSARRGDVPPQQALEEISHTVERATELANQMLALA
KVEQLRQDGRPPVIDWSGVVRAVALDLSALIADRSLDFSLDTQAAPVQAHEWALRELSRN
LLHNAIKNTPADSCLSVHLVVDRQTAALTIRDEGPGISPGLRERLFQPFATDQQRAPGVS
GSGLGLAICREIVLSLDGGIELDNRTHQGQVIGLDAIVRLPLAMAPVTP