Protein Info for GFF5119 in Variovorax sp. SCN45

Annotation: Ammonium transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 80 to 101 (22 residues), see Phobius details amino acids 113 to 133 (21 residues), see Phobius details amino acids 169 to 191 (23 residues), see Phobius details amino acids 198 to 220 (23 residues), see Phobius details amino acids 232 to 253 (22 residues), see Phobius details amino acids 265 to 286 (22 residues), see Phobius details amino acids 296 to 318 (23 residues), see Phobius details amino acids 329 to 349 (21 residues), see Phobius details amino acids 355 to 373 (19 residues), see Phobius details amino acids 385 to 405 (21 residues), see Phobius details amino acids 425 to 447 (23 residues), see Phobius details TIGR00836: ammonium transporter" amino acids 78 to 477 (400 residues), 448.9 bits, see alignment E=8.3e-139 PF00909: Ammonium_transp" amino acids 80 to 477 (398 residues), 412.5 bits, see alignment E=8.4e-128

Best Hits

Swiss-Prot: 62% identical to AMTB_ECO57: Ammonia channel (amtB) from Escherichia coli O157:H7

KEGG orthology group: K03320, ammonium transporter, Amt family (inferred from 83% identity to aaa:Acav_0289)

MetaCyc: 62% identical to ammonium transporter (Escherichia coli K-12 substr. MG1655)
RXN-9615

Predicted SEED Role

"Ammonium transporter" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (479 amino acids)

>GFF5119 Ammonium transporter (Variovorax sp. SCN45)
MKKLLVSLALGLSVLAAGTSGFAQTPAAPAADAPAATAPAAAAAAPAAAPAADAAAPAAA
APAAAPAAAPAPKIDSGDTAWMLTSTLLVILMTIPGLALFYGGLGRSKNMLSVLMQVFVI
FSLISILWAVYGYSLAFSGDGNFFGTFDKIFMKGITQETFGALTTIPEYVFVAFQGTFAA
ITVALIVGAFAERAKFSAVLLFSVLWFTFSYVPIAHIVWGGGLLGKDGALDFAGGTVVHI
NAGVAGLVGAYMVGKRVGYGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSAGAANALAGL
AFINTVLATAAAALSWILGESLHKGKASMLGAASGAVAGLVAVTPAAGFVGPMGSIVLGL
LAGLVCLWGVGGLKRMLGADDAFDVFGVHGVGGILGAILTGVFAAKGLGGTGGATPDTFS
MGAQVWIQIKSVALTVVWSGVVAFIAYKIADLTLGLRVSEEEEREGLDISSHGETAYNR