Protein Info for PS417_25975 in Pseudomonas simiae WCS417
Updated annotation (from data): Acyl-CoA dehydrogenase (EC 1.3.8.7)
Rationale: Specifically important for: Sodium octanoate. presumably part of beta oxidation, but not sure why 2 different ones are required (could relate to different chain lengths) (SEED_correct)
Original annotation: acyl-CoA dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 99% identity to pfs:PFLU5609)Predicted SEED Role
"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)
MetaCyc Pathways
- oleate β-oxidation (30/35 steps found)
- fatty acid salvage (6/6 steps found)
- adipate degradation (5/5 steps found)
- 2-methyl-branched fatty acid β-oxidation (11/14 steps found)
KEGG Metabolic Maps
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Brassinosteroid biosynthesis
- Caprolactam degradation
- Fatty acid metabolism
- Geraniol degradation
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.-
Use Curated BLAST to search for 1.3.8.7 or 1.3.99.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7UPP1 at UniProt or InterPro
Protein Sequence (601 amino acids)
>PS417_25975 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Pseudomonas simiae WCS417) MPDYKAPLRDIRFVRDELLGYEAHYQSLPACQDATPDMVDAILEEGAKFCEQVLAPLNRV GDIEGCTWSESGVKTPTGFKEAYKQFVEGGWPSLAHDVEHGGQGLPESLGLAVSEMVGEA NWSWGMYPGLSHGAMNTISEHGTPEQQEAYLTKLVSGEWTGTMCLTEPHCGTDLGMLRTK AEPQADGTYKVSGTKIFISAGEHDMADNIVHIVLARLPDAPAGTKGISLFIVPKFLPNAD GSIGARNAVTCGSLEHKMGIHGNATCVMNFDAATGFLIGPANKGLNCMFTFMNTARLGTA LQGLAHAEIGFQGGLKYARDRLQMRSLTGPKAPDKAADPIIVHPDVRRMLLTMKAFAEGN RAMVYFTAKQVDIVKYGTDDEAKKQADGLLAFMTPIAKAFMTEVGFESANHGVQIYGGHG FIAEWGMEQNVRDSRISMLYEGTTGIQALDLLGRKVLMTQGEALKGFTKIVHKFCQANEG NEAVKEFVAPLAALNKEWGELTMKVGMAAMKDREEVGAASVDYLMYSGYACLAYFWADMA RLAAEKLAAGTTEEAFYTAKLQTARFYFQRILPRTRTHVATMLSGANNLMDMKEEDFGLA Y