Protein Info for GFF507 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868
Annotation: Cytoplasmic trehalase (EC 3.2.1.28)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to TREF_SALPC: Cytoplasmic trehalase (treF) from Salmonella paratyphi C (strain RKS4594)
KEGG orthology group: K01194, alpha,alpha-trehalase [EC: 3.2.1.28] (inferred from 100% identity to seg:SG3834)MetaCyc: 91% identical to cytoplasmic trehalase (Escherichia coli K-12 substr. MG1655)
Alpha,alpha-trehalase. [EC: 3.2.1.28]
Predicted SEED Role
"Cytoplasmic trehalase (EC 3.2.1.28)" in subsystem Trehalose Uptake and Utilization (EC 3.2.1.28)
MetaCyc Pathways
- trehalose degradation II (cytosolic) (2/2 steps found)
- trehalose degradation VI (periplasmic) (2/2 steps found)
- chitin biosynthesis (6/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.2.1.28
Use Curated BLAST to search for 3.2.1.28
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (549 amino acids)
>GFF507 Cytoplasmic trehalase (EC 3.2.1.28) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868) MLNQKLNPTPSEDLTIDVDLLYETDPCELKLDEMIEAEPEPEMIEGLPASDALTPADRYL ELFEHVQSTKLFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRRFVENHFWLPETLS SEYVSNPENSLKEHIDQLWPILTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR GARRYLDHLKMEYAFWMDGAESLALNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDAGRLASIRTTQFIPIDLNAFLYKLES AIANISALKGERDTEALFRQKASDRRAAVNHYLWDDENGCYRDYDWRREEMALFSAASIV PLYVGMANHEQADRLANVVRSRLLTPGGIMATEYETGEQWDKPNGWAPLQWMAIQGFKRY GDDMLGDEIAHNWLKTVNHFYQEHHKLIEKYHISGGTPREGGGGEYPLQDGFGWTNGVVR RLIGLYGEP