Protein Info for GFF5041 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 6 to 429 (424 residues), 650.4 bits, E=5.3e-200
• PF01053: Cys_Met_Meta_PP
  • amino acids 8 to 428 (421 residues), 450.7 bits, E=6.3e-139
• PF01041: DegT_DnrJ_EryC1
  • amino acids 61 to 185 (125 residues), 35.1 bits, E=1.8e-12
• PF00155: Aminotran_1_2
  • amino acids 64 to 224 (161 residues), 34.6 bits, E=2.6e-12
• PF00266: Aminotran_5
  • amino acids 134 to 217 (84 residues), 25.7 bits, E=1.2e-09

Best Hits

Swiss-Prot: 54% identical to FUB7_FUSO4: Sulfhydrylase FUB7 (FUB7) from Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 85% identity to mpt:Mpe_A0028)

MetaCyc: 50% identical to Met17 (Saccharomyces cerevisiae)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• homocysteine and cysteine interconversion (2/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Protein Sequence (431 amino acids)

>GFF5041 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSADWKFETRSVHAGYRPDPTTKAVAVPIYQTAAYAFDNAQHGADLFDLKVPGNIYTRIM
NPTQDVLEQRIASLEGGIAALALASGQAAVTYAIQTIAESGDNIVSSTALYGGTYNLFAH
TLPQFGITTRFADHRDPASFEPLIDARTKAIFVESIGNPQGNITDIAAVAAIAHKHGVPL
IVDNTVASPYLLRPIEHGADIVVQSLTKYLGGHGTSIGGAIIDSGKFPWGKHKARFKRLN
EPDPSYHGVVYTEALGPAAYIGRARVVPLRNTGAAISPFNAFLILQGIETLSLRLDRINA
NTLRVAKHLEQHAKVGWVNYAGLPSHPDHALALKYLGGHASGLLTFGVKAAAGEGRAAGA
RFLDALQLFTRLVNIGDAKSLATHPASTTHRQLSPEELKKSGVPEDAVRLSIGIEHIDDL
LADLNQALASV