Protein Info for GFF5040 in Variovorax sp. SCN45

Annotation: 3-hydroxypropionate dehydrogenase DddA, flavin-containing (EC 1.1.1.59)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00732: GMC_oxred_N
  • amino acids 6 to 303 (298 residues), 156.5 bits, E=1.4e-49
• PF05199: GMC_oxred_C
  • amino acids 395 to 533 (139 residues), 125.4 bits, E=3.8e-40

Best Hits

KEGG orthology group: K00108, choline dehydrogenase [EC: 1.1.99.1] (inferred from 92% identity to vpe:Varpa_0314)

MetaCyc: 70% identical to 3-hydroxypropionate dehydrogenase (Halomonas sp. HTNK1)
3-hydroxypropionate dehydrogenase. [EC: 1.1.1.59]

Predicted SEED Role

"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)

MetaCyc Pathways

• superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
• choline-O-sulfate degradation (3/3 steps found)
• β-alanine biosynthesis II (5/6 steps found)
• choline degradation I (2/2 steps found)
• glycine betaine biosynthesis I (Gram-negative bacteria) (2/2 steps found)
• glycine betaine biosynthesis II (Gram-positive bacteria) (2/2 steps found)
• acrylate degradation I (3/5 steps found)
• propanoyl-CoA degradation II (3/5 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Propanoate metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.1

Use Curated BLAST to search for 1.1.1.59 or 1.1.99.1

Protein Sequence (543 amino acids)

>GFF5040 3-hydroxypropionate dehydrogenase DddA, flavin-containing (EC 1.1.1.59) (Variovorax sp. SCN45)
MSETQFDYIIIGAGTAGSLMANRLSADKSKRVLLVEAGRKDDYHWIHIPVGYLYCIGNPR
TDWLYSTEPDAGLNGRVLRYPRGKTLGGSSSINGMIYMRGQSRDYEQWARLTGDESWRWQ
NVLPAFKKHEDFYLGADEMHGAGGEWRVEKQRLRWDILDAFAEAAAQADVPHTTDFNRGS
NEGVGYFQVNQKNGWRWNTAKAFLRPTCYGRPNFELWTGAQVSRLLFETQPDGTRRCTGA
EVWNGSEMVTAHASREVILSAGAIGSPQILQLSGIGPAALLRQHGIDVVLDAPGVGANLQ
DHLQIRAVYKISGAPTLNVLASSMFGKAKIGLEYMLRRSGPMSMAPSQLGAFTRSSPDHE
WPNLQYHVQPLSLDAFGDPLHSFPAFTASVCNLNPTSRGAVRIKSGRFQDAPAIAPNYLS
TDEDRKVAADSLRVTRRIASQPALAKYQPQEWKPGAQYQTDEDLARLAGDIATTIFHPVG
TTKMGADGDPMAVLDSRLRVRGVQGLRVVDAGAMPTITSGNTNSPTLMMAEKAAGWICGA
AQD