Protein Info for GFF5039 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Methionine ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 267 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR00363: lipoprotein, YaeC family" amino acids 29 to 266 (238 residues), 271.8 bits, see alignment E=3.3e-85 PF03180: Lipoprotein_9" amino acids 32 to 265 (234 residues), 324.3 bits, see alignment E=1.9e-101

Best Hits

Swiss-Prot: 55% identical to METQ_SALTY: D-methionine-binding lipoprotein MetQ (metQ) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02073, D-methionine transport system substrate-binding protein (inferred from 82% identity to rfr:Rfer_1770)

MetaCyc: 55% identical to L-methionine/D-methionine ABC transporter membrane anchored binding protein (Escherichia coli K-12 substr. MG1655)
RXN0-4522 [EC: 7.4.2.11]; 7.4.2.11 [EC: 7.4.2.11]; TRANS-RXN-383 [EC: 7.4.2.11]; TRANS-RXN0-510 [EC: 7.4.2.11]; TRANS-RXN0-511 [EC: 7.4.2.11]

Predicted SEED Role

"Methionine ABC transporter substrate-binding protein" in subsystem Methionine Biosynthesis or Methionine Degradation or Staphylococcal pathogenicity islands SaPI

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (267 amino acids)

>GFF5039 Methionine ABC transporter substrate-binding protein (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTLNKRQLLQSTLALTLAAAFAAPALAQDKPLKIGVTAGPHAQIFEQVKKIAEKDGLKIQ
IVEFSDYVQPNAALAAGDLDANSYQHKPYLDNQIKDRGYKFTAVGYTVNFPIGIYSKKVK
SLKDLPEGGKVGIPNDPTNGGRVLLVLQDQGLIKLKPEAGLKATPLDVVSNPKKLKFVEL
DAAQLPRSLDDLDASAINTNYALSAGLNPGKDAIAQENAKSPYVNLIAVREADKDKPWVA
KLVKAYQSAEVKAFIQTEFKGSVLAGF