Protein Info for GFF5036 in Variovorax sp. SCN45

Annotation: Opine oxidase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01494: FAD_binding_3
  • amino acids 9 to 40 (32 residues), 24.7 bits, see alignment (E = 2.1e-09)
• PF01266: DAO
  • amino acids 11 to 355 (345 residues), 222.4 bits, E=1.9e-69
• PF13450: NAD_binding_8
  • amino acids 13 to 53 (41 residues), 23.4 bits, 9e-09

Best Hits

KEGG orthology group: K00303, sarcosine oxidase, subunit beta [EC: 1.5.3.1] (inferred from 82% identity to vap:Vapar_0303)

Predicted SEED Role

"Sarcosine oxidase beta subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

• glycine betaine degradation I (5/8 steps found)
• creatinine degradation I (2/4 steps found)
• creatinine degradation II (2/5 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Protein Sequence (387 amino acids)

>GFF5036 Opine oxidase subunit B (Variovorax sp. SCN45)
MSGIRKLQTEVAIVGGGIVGSSAALALRHMGIDVVLLERDFCGSRSSGVNFGGVRRQGRP
VSQLPLAQRAHRIWGRLPELLGTDGEYLRSGHFKIARSEADMASLERYRALSSDFDLGLE
LISAARLRAQCPWLGTRAVGGSLCIEDGQANPRLVSPAFALAAARAGAQVFERHKVDEVA
HDGALFTVRSTHADGTLEVQAPVLLNCAGAWASAIAGQFGEMPPLSARSPLMAVTEPVPR
FMTWSLGVEGGGIYCRQVERGNLVLGGGTGITLDADRARAERDGIATLAAQAVELLPALR
HAHIIRTWGGTEGYLPDRQPVLGASRTTPGLFHGFGFSGAGFQIGPAAGEVLAELARDGR
SDTPIDAFAIERFDEAPVSPSLAASPT