Protein Info for GFF5023 in Variovorax sp. SCN45

Annotation: Ammonium transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 transmembrane" amino acids 15 to 37 (23 residues), see Phobius details amino acids 45 to 70 (26 residues), see Phobius details amino acids 112 to 133 (22 residues), see Phobius details amino acids 140 to 161 (22 residues), see Phobius details amino acids 176 to 197 (22 residues), see Phobius details amino acids 209 to 229 (21 residues), see Phobius details amino acids 241 to 261 (21 residues), see Phobius details amino acids 272 to 290 (19 residues), see Phobius details amino acids 296 to 316 (21 residues), see Phobius details amino acids 328 to 347 (20 residues), see Phobius details amino acids 361 to 387 (27 residues), see Phobius details TIGR00836: ammonium transporter" amino acids 13 to 407 (395 residues), 421 bits, see alignment E=2.5e-130 PF00909: Ammonium_transp" amino acids 15 to 407 (393 residues), 379.8 bits, see alignment E=6.9e-118

Best Hits

KEGG orthology group: K03320, ammonium transporter, Amt family (inferred from 94% identity to vpe:Varpa_0347)

Predicted SEED Role

"Ammonium transporter" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (411 amino acids)

>GFF5023 Ammonium transporter (Variovorax sp. SCN45)
MALAPQASINAADTAWMMTSTALVLLMTLPGIALFYAGMVRRKSVLATMAAVVAVAAAVS
LTWFAVGYSLAFTEGWPWLGGAGRFWFSGFEYLKDAGQVAVSHVAPNVPESVYAMFQLTF
AIITTALVLGALVERMRFSAVLWFALLWSVLVYAPIAHWVWEPGGWLAQMGALDFAGGSV
VHVNAGIAGLVCAYALGPRKGYGREAFEPYSLAFTMMGAGLLWVGWFGFNAGSAVAADGR
AGLAMLVTHIAAAAGAMSWMVGEWIVRRSPSLLGLCSGLVAGLVAITPAAGFVTPLAALA
IGAIAGLACYWGATGLKHLLGADDSLDVFGVHGIGGVVGALLTGVFADARISGATGDVLT
QAIAVGSVAIYSATGTAVVLFLVHVLVGLRVDADSEVLGLDLAQHREHLGS