Protein Info for GFF5014 in Variovorax sp. SCN45

Annotation: Acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 PF02771: Acyl-CoA_dh_N" amino acids 8 to 119 (112 residues), 80.1 bits, see alignment E=3.3e-26 PF02770: Acyl-CoA_dh_M" amino acids 123 to 221 (99 residues), 87.1 bits, see alignment E=1.5e-28 PF00441: Acyl-CoA_dh_1" amino acids 233 to 389 (157 residues), 141.1 bits, see alignment E=6.6e-45 PF08028: Acyl-CoA_dh_2" amino acids 249 to 371 (123 residues), 64.4 bits, see alignment E=2.6e-21

Best Hits

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 92% identity to vpe:Varpa_0339)

Predicted SEED Role

"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2, 1.3.99.3

Use Curated BLAST to search for 1.3.99.2 or 1.3.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (391 amino acids)

>GFF5014 Acyl-CoA dehydrogenase (Variovorax sp. SCN45)
MNAELQADRAALADTVRRFAENEIAPNVQAWDDAGEFPRALYTRAAELGLLGLGYPEALG
GTPASYALKLPAWVALASHGRSGGVLASLFSHNIGLPPVVLHGSDAVREEVAPPVLRGEK
IAALAITEPGGGSDVAALRTTARRVPDGDGDHYVVNGEKTFITSGMRADWITVAVRTGEG
RGAGGISMLLVPGDAAGLSRTRLSKMGWLCSDTATLHFDNVRVPARYLLGNEGEGFRMVM
GNFNGERIGLAAGALGFAQACLDEALAWARERKAFGAALIEHQAVRHKLVDMRMRIASTE
AWIEAVSAEGDALEAAGRFNAPEWVAQICMLKNHATQTMQFCADQAVQILGGMGFMRGTV
SERIYREVKVMMIGGGAEDIMKELAAKQMGW