Protein Info for GFF5011 in Variovorax sp. SCN45

Annotation: Geranyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 PF00289: Biotin_carb_N" amino acids 5 to 113 (109 residues), 159.7 bits, see alignment E=9.8e-51 PF02786: CPSase_L_D2" amino acids 118 to 325 (208 residues), 246.7 bits, see alignment E=5.2e-77 PF07478: Dala_Dala_lig_C" amino acids 145 to 294 (150 residues), 37.4 bits, see alignment E=6.1e-13 PF02785: Biotin_carb_C" amino acids 339 to 443 (105 residues), 95.8 bits, see alignment E=5e-31 PF00364: Biotin_lipoyl" amino acids 584 to 647 (64 residues), 46.9 bits, see alignment E=6e-16

Best Hits

KEGG orthology group: K13777, geranyl-CoA carboxylase alpha subunit [EC: 6.4.1.5] (inferred from 87% identity to vpe:Varpa_0336)

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.5

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (650 amino acids)

>GFF5011 Geranyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.5) (Variovorax sp. SCN45)
MNTNFRKILIANRGEIAVRVMRTARAMGWRTVAVYSTADADAEHVRQADEAVWIGESLPA
QSYLNIAAIVDAARASGADAVHPGYGFLAENASFAQACRDAGLVFIGPSPEAILAMGDKA
GAKRLMQAAGVPCIPGYQGEDQGADRLAAEAARIGWPVMIKATAGGGGRGMRLVASAVGF
AEALQSAQSEALNAFGDATVILERAIVAPRHIEIQVFADRHGNAIHLGERDCSVQRRHQK
VIEEAPSPAVSAALRERMGATAVAAAKAIAYEGAGTLEFLLDAEGQYWFMEMNTRLQVEH
PVTEAVTGLDLVELQLRVAAGEPLPLSQQDVRIDGHAIEVRLCAEDPLQGFMPQSGTLAA
WRPSPALRTEHALRDGAAVPPFYDSMIAKLVASGRTRDEARRKLLAGLGDTVALGVATNQ
QLLQRALSHEVFAGGGATTAFIADHLDALLAPDAATDTQAALLAALLLQLGERGWPSPLA
HTLPNALRFALGGAVHEARVTPRGAGRFDVALDGAPPEPVALLALPGDGTVRFSCDGLSE
QAFVVRDAGRSSFHFRGQAFQLDDLTHAAVARAGADGGDGLLRASMNGRVIALLAAEGDT
VAAGQPLVTLEAMKMEHVHCAPRAGRVAALHVAVGAQVAARHVVAEIADA