Protein Info for GFF5003 in Pseudomonas sp. DMC3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 953 PF00359: PTS_EIIA_2" amino acids 4 to 140 (137 residues), 95.1 bits, see alignment E=1e-30 PF00381: PTS-HPr" amino acids 285 to 366 (82 residues), 62.4 bits, see alignment E=8.3e-21 TIGR01003: phosphocarrier, HPr family" amino acids 285 to 365 (81 residues), 49.2 bits, see alignment E=3.9e-17 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 404 to 951 (548 residues), 495 bits, see alignment E=2.7e-152 PF05524: PEP-utilisers_N" amino acids 404 to 519 (116 residues), 82.5 bits, see alignment E=7e-27 PF00391: PEP-utilizers" amino acids 544 to 616 (73 residues), 77.4 bits, see alignment 1.3e-25 PF02896: PEP-utilizers_C" amino acids 645 to 925 (281 residues), 359.8 bits, see alignment E=2.6e-111

Best Hits

KEGG orthology group: K11189, phosphocarrier protein (inferred from 98% identity to pfo:Pfl01_0793)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (953 amino acids)

>GFF5003 hypothetical protein (Pseudomonas sp. DMC3)
MLELTIEQISMGQSAVDKPAALQLLASHLVADGLVADGYLAGLQAREAQGSTFLGQGIAI
PHGTPETRDQVFATGVRLMQFPDGVDWGDGQIVYLAIGIAAKSDEHLRLLQLLTRALGET
DLGQALRRASSPEALLKLLQGAPQELALDAQMIGLGVSADDFEELVWRGARLLRQADCVS
NGFAGVLQQVEALPLGDGLWWLHSEQTVKRPGLAFVTPDKPMRYLGQPLSGLFCLASLGE
AHQALLERLCALLIEGRGHELGRATSSRKVLEVLGGELPADWPSARIALANAHGLHARPA
KILAQLAKSFDGEIRVRIVDSQDSAVSVKSLSKLLSLGARRGQVLELIAEPSIAADALPA
LLAAIEEGLGEEVEPLPAVSQQREVIADIAEVLIAPASGSLLQAIPAAPGIAIGPAHIQV
LQAIDYPLRGESAAIERERLKQALSDVRRDIQGLIERSKAKAIREIFITHQEMLDDPDLT
DEVDTRLKQGESAEAAWMAVIEAAAKQQESLQDALLAERAADLRDIGRRVLAQLCGVQTA
NEPEQPYILVMDEVGPSDVARLDPARVAGILTARGGATAHSAIVARALGIPALVGAGAAV
LLLEPGTPLLLDGQRGRLHVDADAATLKRAAEERDTREQRLKIAAEQRHQPAHTTDGHAV
EVFANIGESAGVTSAVEQGAEGIGLLRTELIFMAHPQAPDEATQEAEYRRVLDGLAGRPL
VVRTLDVGGDKPLPYWPIAKEENPFLGVRGIRLTLQRPQIMEAQLRALLRSADNRPLRIM
FPMVGSVEEWRQARDMTERLRLEIPVADLQLGIMIEVPSAALLAPVLAKEVDFFSVGTND
LTQYTLAIDRGHPTLSAQADGLHPAVLQLIDITVRAAHAHGKWVGVCGELAADPLAVPVL
VGLGVDELSVSGRSIAEVKARIRELSLTQAQTLAQQALAVGSANEVRALVEAL