Protein Info for GFF494 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Protein yhjK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 668 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 152 to 178 (27 residues), see Phobius details PF17154: GAPES3" amino acids 36 to 154 (119 residues), 158.3 bits, see alignment E=1.2e-50 PF00990: GGDEF" amino acids 247 to 394 (148 residues), 39.6 bits, see alignment E=7.2e-14 PF00563: EAL" amino acids 415 to 648 (234 residues), 223.7 bits, see alignment E=3.6e-70

Best Hits

Swiss-Prot: 84% identical to PDEK_ECOLI: Probable cyclic di-GMP phosphodiesterase PdeK (pdeK) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 90% identity to cko:CKO_04971)

MetaCyc: 84% identical to c-di-GMP phosphodiesterase PdeK (Escherichia coli K-12 substr. MG1655)
Cyclic-guanylate-specific phosphodiesterase. [EC: 3.1.4.52]

Predicted SEED Role

"Protein yhjK"

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.52

Use Curated BLAST to search for 3.1.4.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (668 amino acids)

>GFF494 Protein yhjK (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
LRVSRSLTIKQMAMVAAVVMMFVFVFCTVLLFHLVQQNRYNTATQLESIARSVREPLSSA
ILKADLPGAETILESIKPAGVVSRADVVLPNQFQALRKRFIPERPVPVMVTRLFELPVQI
SLPVYSLERPANPQPLAYLVLQADSYRMYKFVMSALSTLVTIYLLLSLILTVAIAWCVNR
LIVHPLRKIARELNDIPQQELIGHQLALPRLHQDDEIGMLVRSYNLNQQLMQRQREEQTD
NAMRFPVSELPNKAFLMALLEQVITRQQTTALIIVTCETLRDTAGVLQETQREILLLTLV
EKLKSVLAPRMVLTQVSGYDFAIIAHGVKEPWHAITLGQQILTIINERLPIQGIQLRPSC
SIGIAMYYGDLTAEALYGRAVSAAFTARRKGKNQIQFFDPAQMEAAQQRLTEESDILTAL
DNHQFAIWLQPQVEMRSGNVLSAEALLRMQQPDGSWELPEGLIERIESCGLMVTVGYWVL
EESCRQLAAWQERGVTLPLSVNLSALQLMHPGMVSDLLELLNRYRIQPGTLILEVTESRR
IDDPHAAVAILRPLRNAGVRIALDDFGMGYAGLRQLQHMKSLPVDILKIDKMFVDGLPDD
HSMVTAIILMARSLNLQLIAEGVENEAQRAWLEQAGVNVAQGFLFARPVPADIFEERYLS
HENPDYKS