Protein Info for GFF493 in Pseudomonas sp. DMC3

Annotation: Acetylpolyamine amidohydrolase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 341 PF00850: Hist_deacetyl" amino acids 28 to 336 (309 residues), 234 bits, see alignment E=1.2e-73

Best Hits

Swiss-Prot: 71% identical to APAH1_PSEAE: Acetylpolyamine amidohydrolase 1 (aphA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 95% identity to pfo:Pfl01_5631)

MetaCyc: 52% identical to acetylpolyamine amidohydrolase subunit (Mycoplana ramosa)
Acetylputrescine deacetylase. [EC: 3.5.1.62]

Predicted SEED Role

"Deacetylases, including yeast histone deacetylase and acetoin utilization protein" in subsystem Hydantoin metabolism

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.62

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (341 amino acids)

>GFF493 Acetylpolyamine amidohydrolase 1 (Pseudomonas sp. DMC3)
MLTIYSDDHHLHHGRCELIDGQLKPCFEMPSRADHVLQRVQKQNLGAVEAPKDFGLEPIA
RIHSRDYLEFFKGAWERWTEFNTDGDLLPYTWPARTLRAIKPTSLHGQLGYYSFDGGAPI
TAGTWQAAYSAAQVALTAQAEIQRGARAAFALCRPPGHHAASDLMGGYCYLNNAAIAAQA
FLDQGHKKVAILDVDYHHGNGTQSIFYERSDVLFTSIHGHPEAEFPFFLGYDDERGEGAG
EGFNFNYPLPAGSGWDVWSAALDQACDEIARYGADIIVVSLGVDTFKDDPISQFKLDSPD
YLAMGKRIAALGKPTLFVMEGGYAVEEIGINAVNVLEGFEQ