Protein Info for GFF4892 in Xanthobacter sp. DMC5

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 PF01494: FAD_binding_3" amino acids 6 to 185 (180 residues), 29.8 bits, see alignment E=1.5e-10 PF12831: FAD_oxidored" amino acids 7 to 174 (168 residues), 31 bits, see alignment E=6.9e-11 PF00890: FAD_binding_2" amino acids 7 to 43 (37 residues), 26.9 bits, see alignment 1.2e-09 PF07992: Pyr_redox_2" amino acids 7 to 165 (159 residues), 26.9 bits, see alignment E=1.3e-09 PF01134: GIDA" amino acids 7 to 165 (159 residues), 27.8 bits, see alignment E=5.3e-10 PF13450: NAD_binding_8" amino acids 10 to 41 (32 residues), 23.7 bits, see alignment (E = 2e-08) PF21162: ETFQO_UQ-bd" amino acids 206 to 283 (78 residues), 29 bits, see alignment E=5.1e-10

Best Hits

Swiss-Prot: 82% identical to FIXC_AZOC5: Protein FixC (fixC) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K00313, electron transfer flavoprotein-quinone oxidoreductase [EC: 1.5.5.-] (inferred from 90% identity to xau:Xaut_0140)

MetaCyc: 60% identical to quinone reductase (NADH,flavodoxin) complex electron transfer catalytic subunit (Azotobacter vinelandii)
1.19.1.M1 [EC: 1.19.1.M1]

Predicted SEED Role

"Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 1.5.5.-)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.19.1.M1 or 1.5.5.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (435 amino acids)

>GFF4892 hypothetical protein (Xanthobacter sp. DMC5)
MIDERFDAIVVGAGMAGNAAAYTMASRGLKVLQLERGEYSGSKNVQGAILYADMMEKIIP
DFREDAPLERHLIEQRFWMMGENSHTGLHYRSDDFNEEKPNRYTIIRAQFDKWFSSKVRE
AGALVVCETTVTELVQDAYGKVIGVKTDRGGGQIHADVVVLAEGVTGLLGARAGLRKIPK
PTEVALAVKEMHFMPQEVIEQRFNVKGNEGAVIEAAGTISEGMTGMGFLYTNRECVSVGI
GCLVSDFQKSGESPYTLLEKFKRHPSIAPLLEGSEVKEYAAHLIPEGGFKSIPQLYGEGW
VVVGDAAQLNNAIHREGSNLAMTSGRIAGEAIFQVKSRKDPMTASNLSLYKKMLDDSFVI
KDLKKYKDMPQLLHIQSQNFFLTYPQLVNKAMQNFVRVDGTPKKEKEKMTVKSFTSARSW
AGLFGDAFKLARAWR