Protein Info for GFF489 in Pseudomonas sp. DMC3

Annotation: Amino-acid carrier protein AlsT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 482 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 60 to 83 (24 residues), see Phobius details amino acids 89 to 112 (24 residues), see Phobius details amino acids 139 to 158 (20 residues), see Phobius details amino acids 177 to 195 (19 residues), see Phobius details amino acids 204 to 223 (20 residues), see Phobius details amino acids 236 to 256 (21 residues), see Phobius details amino acids 291 to 315 (25 residues), see Phobius details amino acids 341 to 361 (21 residues), see Phobius details amino acids 374 to 392 (19 residues), see Phobius details amino acids 398 to 421 (24 residues), see Phobius details TIGR00835: amino acid carrier protein" amino acids 13 to 431 (419 residues), 465 bits, see alignment E=1.2e-143 PF01235: Na_Ala_symp" amino acids 49 to 444 (396 residues), 499.7 bits, see alignment E=3.9e-154

Best Hits

Swiss-Prot: 44% identical to ALST_BACSU: Amino-acid carrier protein AlsT (alsT) from Bacillus subtilis (strain 168)

KEGG orthology group: K03310, alanine or glycine:cation symporter, AGCS family (inferred from 94% identity to pfo:Pfl01_5626)

Predicted SEED Role

"sodium/alanine transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (482 amino acids)

>GFF489 Amino-acid carrier protein AlsT (Pseudomonas sp. DMC3)
MLEVINDFLSGKVLIVLIVGLGSYFTIRSRFVQLRHFFHMFAVFRDSLKGSAGQLSSFQA
LMLSLAGRVGAGNIAGVGIAVTLGGPGAVFWMWVTALVGMSSSFFECTLAQVYKRADGDG
LYRGGPAYYIQHGLKLKGMAIVFSVLLLVTYGFAFIGLQSYTVTHSLQNAFDFNPQHTGI
VLAVLLAITFIGGIKRIASVSDLLVPIKTLAYIGVTLYVIGTQIEHVPAMLETIFKSAFG
LDPAFAGLLGSAIVMGVKRGVFANEAGLGSAPNVAAVAAVKHPGAQGVVQAFSVFLDTFV
ICTCTALLILLSGFYTPGFEGDGIVLTQNSLAAVVGDWGRMFVSVALSLFVFTCILYNYY
LGENSLQFLSRNRALLMLFRGLVLALVVWGSMQDLTTVFAFADITMTCLAFVNLVALAML
FKVGLRVMRDYDEQRRAGVKQPVFDSSKFADLDLDRNAWPVNPPAAAGQTETAPQGVPAA
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