Protein Info for GFF4878 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 963 PF16078: 2-oxogl_dehyd_N" amino acids 19 to 56 (38 residues), 61.1 bits, see alignment (E = 1.2e-20) TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component" amino acids 21 to 952 (932 residues), 1216.1 bits, see alignment E=0 PF00676: E1_dh" amino acids 228 to 512 (285 residues), 136.1 bits, see alignment E=2.7e-43 PF02779: Transket_pyr" amino acids 607 to 803 (197 residues), 162.5 bits, see alignment E=1.6e-51 PF16870: OxoGdeHyase_C" amino acids 809 to 953 (145 residues), 186.1 bits, see alignment E=6.9e-59

Best Hits

Swiss-Prot: 76% identical to ODO1_CUPNH: 2-oxoglutarate dehydrogenase E1 component (odhA) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 87% identity to aaa:Acav_2000)

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (963 amino acids)

>GFF4878 2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSDSQSNGGNVYVAYNGNSYLFGGNAPYVEEMYENYLDNPGSVPDNWRGYFDALQNVPAG
DGSDARDVPHQPVINAFAERAKQGGTKVVVAAGADSELARKRTAVQQLIAAYRNVGARWA
DLDPLKRAERDHIPELELSFYGFTDADLETVFNTSNTFFGKETMSLRELLNALRETYCGT
IGAEYMYITDQSQKRWWQERLEATRTKPVFNADKKKHILDRLTAAEGLERFLHTKYVGQK
RFSLEGGESFIASMDELITQAGQIGVQEIVIGMAHRGRLNVLVNTLGKMPKDLFAEFDHT
APEELPSGDVKYHQGFSSDVTTPGGPVHLSLAFNPSHLEIVNPVVEGSVRARMDRRGDPL
GKQVLPVLVHGDAAFAGQGVVMETLALAETRGYSTGGTVHLVINNQIGFTTSDPRDSRST
LYCSDVVKMIEAPVLHVNGDDPEAVVFATKLALDFRMTFQKDVVVDIVCFRKLGHNEQDT
PSLTQPLMYKKIAAHPGTRKLYADKLAAQGLGETLGDDMVKAYRAALDAGRHTVDPVLTN
FKSKFAVDWSPYLGKKWTDSADSAIPMAEWKRLSDRLTTIPDNVTPHQLVKKVYADRAAM
GRGDIPVDWGMGEHMAFASLVASGFPVRLSGEDCGRGTFTHRHAVIHDQNREKYDEGIYV
PLQNVADNQAPFVVIDSILSEEAVLGFEYGYASNDPNTLVIWEAQFGDFVNGAQVVIDQF
IASGEVKWGRVNGITMMLPHGYEGQGPEHSSARLERFMQLAADTNMQVVQPTTASQIFHV
LRRQMIRGLRKPLIIFTPKSLLRNKDATSPLSEFTKGGFQTVIPEQNADVVKKAEKVKRV
ICCSGKVYYDLVKKREERGSDDVAIVRVEQLYPFPHKVFGAELRKYPNATDIVWCQDEPQ
NQGAWFFVQHYIHENMLEGQKLGYAGRAASASPAVGYAHLHQEQQKTLIEAAFGKLKGFI
LTK