Protein Info for GFF487 in Variovorax sp. SCN45

Annotation: ATP-dependent protease La (EC 3.4.21.53) Type I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 813 PF02190: LON_substr_bdg" amino acids 14 to 206 (193 residues), 197 bits, see alignment E=8.7e-62 TIGR00763: endopeptidase La" amino acids 15 to 775 (761 residues), 965 bits, see alignment E=1.9e-294 PF00004: AAA" amino acids 356 to 494 (139 residues), 73.8 bits, see alignment E=4.8e-24 PF07728: AAA_5" amino acids 356 to 489 (134 residues), 38.7 bits, see alignment E=2.4e-13 PF05362: Lon_C" amino acids 574 to 776 (203 residues), 315.3 bits, see alignment E=4.2e-98 PF13541: ChlI" amino acids 624 to 744 (121 residues), 27.7 bits, see alignment E=5.6e-10

Best Hits

Swiss-Prot: 66% identical to LON_ECOLI: Lon protease (lon) from Escherichia coli (strain K12)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 88% identity to aav:Aave_1457)

MetaCyc: 66% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (813 amino acids)

>GFF487 ATP-dependent protease La (EC 3.4.21.53) Type I (Variovorax sp. SCN45)
MSGHTPLPPEALDLPLLPLRDVVVFPHMVIPLFVGRPKSIKALELAMEAERRIMLVAQKA
AAKDEPSVEDMFEVGCVSTILQMLKLPDGTVKVLVEGQQRARVNRIDDGETHFSANVTPV
EAVEGGEKGTEVEALRRAVMQQFDQYVKLNKKIPPEILTSISSIDDPGRLADTIAAHLPL
KLDNKQAVLDLDDVKARLENLFGQLEREVDILNVDKKIRGRVKRQMEKNQRDFYLNEQVK
AIQKELGEGEEGADIEEIEKKIKLAKMSKEGLKKAEGELKKLKLMSPMSAEATVVRNYID
VLIGLPWSKKTKIKHDLANAEAVLNADHYGLEKVKDRILEYLAVQQRVDKVKAPILCLVG
PPGVGKTSLGQSIAKATGRKYTRMALGGMRDEAEIRGHRRTYIGALPGKVLQGLSKIGTR
NPLFLLDEIDKLGTDFRGDPSSALLEVLDPEQNHTFGDHYVEVDFDLSDVMFVATSNSMN
IPPALLDRMEVIRLSGYTEDEKTNIAIKYLLPKQMKNNGVKDEELFVTEEAVRDIVRYYT
REAGVRSLERELSKICRKVVKGLLLKQLTPKVTVDGANLNEFLGVRKYSFGLAEKQNQVG
QVVGLAWTEVGGDLLTIEAVTMPGKGVISRTGSLGDVMKESVEAARTVVRSRSRRLGIKD
EVFEKRDIHIHVPDGATPKDGPSAGAAMTTAFVSALTGIPVRSDVAMTGEITLRGEVTAI
GGLKEKLLAALRGGIKTVLIPEENAKDLQDIPENVKNGLEIVPVKWIDKVLEIALEKMPE
ELSDEEVAASAAAVAELAKQRAAAPASGGSVKH