Protein Info for GFF4862 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Histidyl-tRNA synthetase (EC 6.1.1.21)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 TIGR00442: histidine--tRNA ligase" amino acids 7 to 404 (398 residues), 483.4 bits, see alignment E=3e-149 PF13393: tRNA-synt_His" amino acids 10 to 312 (303 residues), 160.6 bits, see alignment E=8.4e-51 PF00587: tRNA-synt_2b" amino acids 68 to 319 (252 residues), 63.4 bits, see alignment E=4.4e-21 PF03129: HGTP_anticodon" amino acids 343 to 403 (61 residues), 23.7 bits, see alignment E=6.4e-09

Best Hits

Swiss-Prot: 72% identical to SYH_ACISJ: Histidine--tRNA ligase (hisS) from Acidovorax sp. (strain JS42)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 72% identity to ajs:Ajs_1171)

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>GFF4862 Histidyl-tRNA synthetase (EC 6.1.1.21) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MADKLSAVKGMNDIAPPASAHWEWLEDTLRTLMRRYGYRNLRTPIVEPTALFVRGLGEVT
DIVEKEMYSFEDRLNGEQLTLRPENTAGVVRAALEHSMLYDGGKRLYYMGPMFRHERPQR
GRYRQFHQFGAEALGFAGPDVDAELIVLACDLWAELGLTGIELEINSLGQPPERLAHRQA
LIAYLEAHADQLDEEAKRRLYSNPLRILDTKNPAMQDLVNAAPRLMDHLGAESLDHFNRL
RGLLDAHGIAYRINPRLVRGMDYYNLSVFEFVTTQLGSQGTVCAGGRYDGLFEQIGGKAA
PAVGWAMGMERILELLKEQGLLPEQPAPDAYAVVPEAARVPQVMPVLRALRQQGVNVQMH
AGGADGMGSMKSQFKKADASGARFALIFGEHEMAQGAVALKPLRGPRTDAPAEAAQSLLP
LDAIADWAGRLRAA