Protein Info for GFF4861 in Pseudomonas sp. DMC3

Annotation: Sensor histidine kinase RcsC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1378 PF01339: CheB_methylest" amino acids 27 to 202 (176 residues), 164 bits, see alignment E=1.8e-51 PF03705: CheR_N" amino acids 242 to 288 (47 residues), 51.2 bits, see alignment (E = 4.8e-17) PF01739: CheR" amino acids 304 to 494 (191 residues), 194.5 bits, see alignment E=8.3e-61 PF13596: PAS_10" amino acids 756 to 860 (105 residues), 86.7 bits, see alignment 9.5e-28 TIGR00229: PAS domain S-box protein" amino acids 874 to 994 (121 residues), 61 bits, see alignment E=6.4e-21 PF13188: PAS_8" amino acids 875 to 918 (44 residues), 19.1 bits, see alignment (E = 5.7e-07) PF00989: PAS" amino acids 877 to 969 (93 residues), 23.9 bits, see alignment 2.2e-08 PF13426: PAS_9" amino acids 888 to 987 (100 residues), 35.4 bits, see alignment 6.6e-12 PF00512: HisKA" amino acids 1010 to 1077 (68 residues), 52.8 bits, see alignment 2e-17 PF02518: HATPase_c" amino acids 1122 to 1234 (113 residues), 88.3 bits, see alignment E=2.8e-28 PF00072: Response_reg" amino acids 1258 to 1369 (112 residues), 81.6 bits, see alignment E=2.9e-26

Best Hits

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80

Use Curated BLAST to search for 2.1.1.80

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1378 amino acids)

>GFF4861 Sensor histidine kinase RcsC (Pseudomonas sp. DMC3)
MTSKTPASPSAPERKDLTPSHLNFPVVGIGASAGGLQATKLFFEHMPKDSGMAFVIILHL
SPDHQSIADQIIQESTRMPVLQVTQPVPIEKNCVYVISPAHSLTMNDGYLRVSPAGSPQA
RHTAIDLFFRDLADVHRERAFCLVLSGTGSDGAVGLSRIKEQGGVTLVQVPEDAEFDGMP
RAAIETRMVDLILPVVEMPQKLMELWRNSQNIKLPSANDPDLKALKPANEHEASIAEQRL
HEILLYLRSSTGHDFKHYKRATVLRRIERRMQVTSQSDLGAYYDYLQNTPEESKALLGDM
LIGVTNFFRDREAFEALERDVLPQLVQSVADSSEKEELRIWSAGCSTGEEAYSLAILLDD
QMQVQSSKANLQVFATDIDERAITTGRAGTYPQAIVTDVPPTRLRHYFVKDNEHYRIRKE
IREKVLFAKHSLLSDPPFSQIDLIVCRNLLIYLDREVQREILQMFHFALRPGGFLFLGTS
ESADACHELFAPVDKRNRIFRAKTGTANSRRTPTMPRGGYMRTSVSRPPSKAIEPVKLSF
ADIHRRAIERFAPPSIIVDTNADILHMSEAAGRFLRHVGGELSRNLLTLILPELRLEIRT
TLFQVQQSNLTVRSREVLLKREERQYRITLIAQPYKDDDFDSECVLVTFDETEIDAHEQA
LTTVLQTESQVLSNLERELQRTKLHLQDTIEQSEISSEELKASNEEMQAINEELRSATEE
LETSKEELQSINEELLTVNYELKTKVEETDKINDYLTNLIASTDIATVFVDRSMRIKWFT
PRATDIFSMLPVDTGRSLLDITHRLNYPAMTEDAAQVFESLNMIEREVSSTDNRWYIARL
LPYRSSEDHIDGTVLTFIDISKRREAEEELRLGEERMRLVAESTRDYAIIILDEMGAITS
WNKGAELIFGHSKNEAEGQYYDFIFTAEDRAAGMAESELSTARQHGRSEDERWHVRKDGS
RFFCSGEVTLLSGDYLQGYVKIARDLTGHKRAQDEQSKELAQTRNTVSLRDEFFAVMSHE
LKHPLNLIQLNAELLRRLPMIRTAGPATKAVNTICDAVNSQARIIDDLLDVARVRTGKLK
LQLARVDLSAVLRDIHTVVLNEQHATRIDLQLSEAPLIIHADPTRVEQIIWNLVNNALKF
TPPSGRIELIASKSGKMAQLDVKDNGVGIAAENLEHVFDLFGQAEKQHTNHQREGLGIGL
SLVRQLTEAQHGTVKVASPGLGAGCTFTVRLPLSTDVEETAATAEVEPVSGKLDGLSILL
VDDSPEVLETLKLLLEMEDADVTAFDHPVAALKAAESTRFDLIISDLGMPVMTGYELMAA
MRKLPLVKNVPAIALTGYGTQSDMQKSREAGFDQHLGKPVAYDDLIETIEALRRSELL