Protein Info for GFF4854 in Sphingobium sp. HT1-2

Annotation: Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1105 TIGR00594: DNA polymerase III, alpha subunit" amino acids 8 to 989 (982 residues), 518.6 bits, see alignment E=1.9e-159 PF02811: PHP" amino acids 11 to 137 (127 residues), 49.2 bits, see alignment E=1.9e-16 PF07733: DNA_pol3_alpha" amino acids 280 to 534 (255 residues), 281.5 bits, see alignment E=1.9e-87 PF17657: DNA_pol3_finger" amino acids 537 to 702 (166 residues), 185.8 bits, see alignment E=1e-58 PF14579: HHH_6" amino acids 776 to 865 (90 residues), 61.3 bits, see alignment E=2.1e-20 PF01336: tRNA_anti-codon" amino acids 956 to 1027 (72 residues), 27.7 bits, see alignment 5.3e-10

Best Hits

Swiss-Prot: 63% identical to DNE21_AGRFC: Error-prone DNA polymerase 1 (dnaE2-1) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 67% identity to oan:Oant_3147)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1105 amino acids)

>GFF4854 Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7) (Sphingobium sp. HT1-2)
MSAGKPGYVELQVTTHFSFLRGASSPEDLFATAAAMEMPALGVVDRHSVAGIVRAWDAER
QTGVRAIVGCRLDLTDGTALLVYPTDKAAYGRLCRLLSIGKTRAGKGACHLDWSDVADWN
EGLLAMLVPDRADATTQAALARTKRLFGERTYMALSVRRRPKDAIRLRDLSRIAAAAGVP
TIATNDVLYHVPERRQLQDVVTCIREKCTIDTLGRTRERFADRYLKTGAEMTRLFRRYLK
DSSPVARSVEFARRCAFSLDELKYQYPDEIQVPGRTPQEELERLTWEKAPMRYPQGVDDK
VRRQLEHELQLIGQLDYAPYFLTVHAIVAEARRREILCQGRGSAANSAVCYVLGITSIDP
VRSELLFERFVSAERREPPDIDVDFEHERREEVIQWIYETYGRTRSALTAVVTRFRARGA
VREVGKALGLSEDVTAGLAGAIWGYSREGVEEKHAQELNLDLSDTRLALTLDLARQLIDT
PRHLSQHPGGFVLTRDRLDELVPIEPAAMDDRQVIEWDKDDIDLLGFMKVDVLALGMLSC
MRRAFEFLENDKGLRHDLATIPAEDPATYAMIRRADTLGVFQIESRAQMASIPLMAPKTF
YDLVIQVAIVRPGPIQGDMVHPYRRRRNGEEEVTYPTEELRRVLEKTLGVPLFQEQAMRV
AIECAGFTASEADLLRRAMATFKLTGGVSDFRDKLISGMVSRGYDQEFAEKTFKQIEGFG
SYGFPESHAASFALIAYASSWMKCHHPDAFCASLLNAQPMGFYAPAQIVRDAREHGVEIR
PIDVNASRWDCTLEEGRGRYKAVRLGLRMARDLANADAAAIVTARGDRPFTSIEEIQQRA
GVGRGALDRIGDADGFGSLGADRRSGLWAVKGLGNAALPLFAAADERAGKLREEAIEPTV
ILAEMGEGAEVVEDYRASGLSLRAHPVAFLREELKARRMITCEQLRTTRDGRWIELAGLV
LVRQKPGSAKGVMFITLEDETDLANLVVWTNVFEKNRRTVLGASMMGVRGQVQREGEVIH
VIAQRLDDLSGMLASVGRRADVADIYRVSRADIARNPTAPDPRDRQQRPLGRDARDIYIP
DLRLGSGIIPGQPTEGIKIKPRDFR