Protein Info for GFF4847 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Arylesterase precursor (EC 3.1.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00657: Lipase_GDSL
  • amino acids 43 to 212 (170 residues), 70.8 bits, E=1.8e-23
• PF13472: Lipase_GDSL_2
  • amino acids 45 to 207 (163 residues), 98.9 bits, E=5.3e-32

Best Hits

Swiss-Prot: 55% identical to EST_PSEAE: Esterase TesA (tesA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K10804, acyl-CoA thioesterase I [EC: 3.1.1.5 3.1.2.-] (inferred from 69% identity to pol:Bpro_3031)

Predicted SEED Role

"Arylesterase precursor (EC 3.1.1.2)" (EC 3.1.1.2)

MetaCyc Pathways

• phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Bisphenol A degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Limonene and pinene degradation
• Ubiquinone and menaquinone biosynthesis
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5, 3.1.2.-

Use Curated BLAST to search for 3.1.1.2 or 3.1.1.5 or 3.1.2.-

Protein Sequence (226 amino acids)

>GFF4847 Arylesterase precursor (EC 3.1.1.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MEGLLKLKRRHFNVLAGALCALAAAGSSGFTHAQATASADPLILVVGDSLSAEYGLKRGT
GWVALLEERLKQEKVRARVVNASISGDTTSGGRSRLAPLLKQHQPALVVIELGGNDALRG
LPLTMTRDNLAAMARLAREAGAKVLLVGMEMPPNYGAKYGQEFREVFTTVAKAEKTALVP
FFLKGVADTKDPTALFQADRIHPNESAQATMRDNVWPELRKLLAAR