Protein Info for GFF4845 in Xanthobacter sp. DMC5

Annotation: Light-independent protochlorophyllide reductase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 TIGR01283: nitrogenase MoFe cofactor biosynthesis protein NifE" amino acids 6 to 458 (453 residues), 664.1 bits, see alignment E=4.6e-204 PF00148: Oxidored_nitro" amino acids 45 to 444 (400 residues), 356.4 bits, see alignment E=2e-110 PF04324: Fer2_BFD" amino acids 496 to 544 (49 residues), 55.8 bits, see alignment 4.6e-19 amino acids 568 to 616 (49 residues), 58.8 bits, see alignment 5.6e-20

Best Hits

Swiss-Prot: 76% identical to NIFE_BRADU: Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE (nifE) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02587, nitrogenase molybdenum-cofactor synthesis protein NifE (inferred from 92% identity to xau:Xaut_0091)

Predicted SEED Role

"Nitrogenase FeMo-cofactor scaffold and assembly protein NifE" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (631 amino acids)

>GFF4845 Light-independent protochlorophyllide reductase subunit B (Xanthobacter sp. DMC5)
MSSVSATIQQVFNEPGCAKNQNKSEAEKKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDV
AHLVHGPIACEGNSWDNRGARSSGSQIWRTGFTTDINETDVVFGGEKRLFKSIKEIIEKY
NPPAVFVYQTCVPAMIGDDIDAVCKAAKEKFGTPVIPINSPGFVGPKNLGNKLAGEAILE
HVIGTEEPEYTTPYDINIIGEYNLSGELWQVKPLLDELGIRILACISGDGRYKDVASSHR
AKAAMMVCSKAMINVARKMEERYDIPFFEGSFYGIQDSSDSLREIARMLIERGADPELMD
RTEALIEREEAKAWAAIAKFKDRFKDKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKS
TKEDKERIKELMGQDAHMIDDMTPREMYKMLKDAKADIMLSGGRSQFIALKAAMPWLDIN
QERHHAYMGYIGMVKLVEEIDKALYNPIWEQVRKPAPWDNPANNWQNRAIAQMEAEAAAL
AADPVAAEKARRAKKICNCKSVDLGTIEDAIKAHSLTTVEGVKTHTNASGGCGACSGRIE
DIFEALGVAEPAPADPVLAEAARRAKKVCNCKSVTVGTIEDAVAAGASNLAEVTARTEAG
SGCGKCGERIEDMLDELVAPLGTPAALVAAE