Protein Info for GFF4826 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 717 transmembrane" amino acids 321 to 337 (17 residues), see Phobius details PF00378: ECH_1" amino acids 9 to 210 (202 residues), 97.6 bits, see alignment E=1.6e-31 PF16113: ECH_2" amino acids 24 to 188 (165 residues), 44.3 bits, see alignment E=3.4e-15 PF02737: 3HCDH_N" amino acids 320 to 498 (179 residues), 183.8 bits, see alignment E=6.1e-58 PF00725: 3HCDH" amino acids 501 to 601 (101 residues), 49.7 bits, see alignment E=9.1e-17

Best Hits

KEGG orthology group: K01782, 3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase [EC: 1.1.1.35 4.2.1.17 5.1.2.3] (inferred from 73% identity to pol:Bpro_3016)

Predicted SEED Role

"Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Isobutyryl-CoA to Propionyl-CoA Module (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.1.2.3

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (717 amino acids)

>GFF4826 Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKTIRYELADGIATITFDEEGSPVNTMCADWQRDLTEVTAQVMHDCADIKGIILASAKST
FFAGADLKGTMRLQPGDAPRVFAEIEQTKKNFRTLETLGVPVVSCINGTALGGGWEVALV
GHHRVAVNDPKIQLGLPEITLGLIPGASGITKMVRLLGLMGAQPYILESKLFNPREAQEL
GLVHELVSPEGDVAHALRNAALDWIAAHPQSRQPWDTKDHKIPGGTPSNPKVAGMLTVAP
AMLKQKTRGLYPAPEAALAAMAEGAMVDFDTALRIESRYLAKLIVSPVAKNMINTFFFNL
NAIKSGQSRPKGISRYKPKKVGVLGAGMMGAGIAYVQAGRGIHTVLEDVSIETAERGKSY
SFKLTQTRVDQGRMSPYDQRELLDRILPTDHPSDLKGCDLIIEAVFENRELKAAVTKETE
PMLAAGGFFASNTSTLPISGLAQASSRPQRFVGIHFFSPVDKMKLVEIIRGKETDDETIA
RAFDYVQALGKLPIVVNDSRGFYTSRTFGTFVMEGAAMLGEGIPAPVIENAAMQAGMPVG
PLAVLDETALSLSVHVMDQTRADFEKEGRRYTATPGELLVERMVKEFKRPGRAGGGGFYD
YPAGQKKQLWPELKARFEQPGTAWNLQDVKDRLIFRQSVETARCLAEGVLTTVHDGNIGS
IFGIGFPAWTGGALQFIYGMGIDAFERRCAELAAHHGAGFALDEATRNAIRQHQPVY