Protein Info for GFF4824 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 PF02771: Acyl-CoA_dh_N" amino acids 9 to 119 (111 residues), 134.2 bits, see alignment E=5.5e-43 PF02770: Acyl-CoA_dh_M" amino acids 123 to 219 (97 residues), 91.4 bits, see alignment E=6.5e-30 PF00441: Acyl-CoA_dh_1" amino acids 234 to 382 (149 residues), 156.3 bits, see alignment E=1.4e-49 PF08028: Acyl-CoA_dh_2" amino acids 251 to 371 (121 residues), 52.3 bits, see alignment E=1.4e-17

Best Hits

Swiss-Prot: 54% identical to ACADL_MOUSE: Long-chain specific acyl-CoA dehydrogenase, mitochondrial (Acadl) from Mus musculus

KEGG orthology group: None (inferred from 85% identity to hmg:100204717)

MetaCyc: 52% identical to long-chain-acyl-CoA dehydrogenase monomer (Homo sapiens)
LONG-CHAIN-ACYL-COA-DEHYDROGENASE-RXN [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-21775 [EC: 1.3.8.8, 1.3.8.7]

Predicted SEED Role

"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.2

Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (387 amino acids)

>GFF4824 Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MIERTLFNADHEAFRDSFRKFVEKEIAPHHEAWEEQGYVDRAVWSKAGENGFLCMTLPEA
YGGSEADKLYSVVQMEEIARSGFSGIGFGLHSEIVAPYILHYGTEEQKKKYLPRLATGEM
VGAIAMSEPAAGSDLQGVKSTAIQQPDGSYLLNGSKTFITNGWHADLVIVVAKTDPGAGA
KGTSLLLVERGMPGFEKGKRLKKLGMKAQDTSELFFDNVKVPAENLLGGPAMEGRGFICL
MEQLPWERLQIAIGAVASAQAAIDWTVQYTKDRKVFGQAVAQYQNTRYTLAELQTEVQVA
RVFVDKCCELIVDGKLDTATASMAKYWCSDLQCKVMDECLQLHGGYGYMWEYPITRAYAD
ARVQRIYGGTNEIMKEVISRGMGLGGR