Protein Info for GFF481 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 5 to 118 (114 residues), 103.3 bits, E=2.1e-33
• PF02770: Acyl-CoA_dh_M
  • amino acids 122 to 222 (101 residues), 69.2 bits, E=5.9e-23
• PF00441: Acyl-CoA_dh_1
  • amino acids 234 to 381 (148 residues), 133.9 bits, E=1.1e-42
• PF08028: Acyl-CoA_dh_2
  • amino acids 265 to 362 (98 residues), 33.1 bits, E=1.3e-11

Best Hits

KEGG orthology group: K11731, citronellyl-CoA dehydrogenase [EC: 1.3.99.-] (inferred from 77% identity to ctt:CtCNB1_1719)

MetaCyc: 59% identical to citronellyl-CoA dehydrogenase (Pseudomonas aeruginosa PAO1)
1.3.8.-

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)" (EC 1.3.8.4)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• citronellol degradation (1/4 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Brassinosteroid biosynthesis
• Caprolactam degradation
• Fatty acid metabolism
• Geraniol degradation
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.4, 1.3.99.-

Use Curated BLAST to search for 1.3.8.4 or 1.3.99.-

Protein Sequence (390 amino acids)

>GFF481 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MQFSHEHREVQKTLKRYIDEHINPHVDEWEAAEMMPAHQIFKGLGDLGLLGLTKPEAYGG
AGLDYSYSMAMAETLGHIHCGGVPMAIGVQTDMATPALARFGSEELKREFLAPSIAGDLV
GCIGVSEPGAGSDVSAIKSVARKDGDDYLISGQKMWITNSLQADWMCMLVNTGDGPAHKN
KSLVMVRMRENGKLVKGIEVAQKIKKIGMNSSDTGLIYFDEVRVPQRNLIGTEGSGFIYQ
MQQFQEERLWCAASTLQSLTNCIGWTVEWAQDRKLFGTSLADQQWVQFKLAELKTEVESL
RALTYLAVEHYVAGEDVTEWATMAKLKAGRLNRIVPDTCLQFWGGMGFTWENKVSRMYRD
GRLASIGGGADEVMMGILSKIMGIAKRPQK