Protein Info for PS417_24550 in Pseudomonas simiae WCS417

Annotation: ATP-dependent helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 836 TIGR01970: ATP-dependent helicase HrpB" amino acids 4 to 834 (831 residues), 1257.2 bits, see alignment E=0 PF04851: ResIII" amino acids 16 to 163 (148 residues), 22.8 bits, see alignment E=1.9e-08 PF00270: DEAD" amino acids 16 to 163 (148 residues), 38.3 bits, see alignment E=2.9e-13 PF00271: Helicase_C" amino acids 207 to 333 (127 residues), 50.9 bits, see alignment E=4.3e-17 PF24473: CON_HrpB" amino acids 591 to 625 (35 residues), 50.6 bits, see alignment (E = 3.4e-17) PF08482: HrpB_C" amino acids 691 to 822 (132 residues), 184 bits, see alignment E=3.9e-58

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 96% identity to pfs:PFLU5389)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1SRW7 at UniProt or InterPro

Protein Sequence (836 amino acids)

>PS417_24550 ATP-dependent helicase (Pseudomonas simiae WCS417)
MNSLPIDEVLPALRDALANRHEAVLEAPPGAGKTTRVPLALLNEPWLAGQTILMLEPRRL
AARAAAERLASELGEKVGETVGYRIRLDSKVGPNTRIEVVTEGILTRRLQDDPALDGVGL
LIFDEFHERSLDADLALALSLNGRELFRDDQPLKILLMSATLEGERLAGLLDNAPILRSE
GRMFPVQMRWGRPYQPGEFIEPRVVQTVLDALQDETGSVLVFLPGQAEIRRVNQQLADAL
GERADVVLCPLHGELDLNAQRAAIDPAPVGKRKVVLATNIAETSLTINGVRVVIDAGLAR
VPRFDPGSGMTRLDTQRISRASATQRAGRAGRLEPGVCYRLWSEDQHEGLAAYGSAEILA
ADLAGLALQLARWGVTPTQLVWLDVPPTAAYAQAQDLLGRLGALDEEKLTAHGQKMAELP
AHPRIAHLLLRGQALGLAATACDVAALLGERDILRGGGADLHSRLALLSGEERARGTQGG
VQRAKQLARQYRGYLRGQATQPVADPDHPRWLGALLALAYPDRVAQQRRPGGAEYRLANG
RAALFAEADSLMKQAWLVIADLGSRQGQREERIYLAADFDPALFDTVLAEQVRNVDQLDW
DEREGVLRAERQRKVGELVLSREPLTGLDESARSQALVNLVRRKGLELLPWTPELRQWQA
RVMLLRQLDAGKTSEWPDLSDKALLASLEHWLMPYLGKVSRLSHFANLDISSFLHNLLPW
PLPQRLDELAPQHVKVPSGSSVRLDYSEQPPILAVRLQELFGLADTPRIASGRVAVKLHL
LSPARRPVQVTQDLANFWRSTYAEVKKDLKGRYPKHYWPDDPLVAEATARIKPRKP