Protein Info for GFF4791 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: tRNA dihydrouridine synthase A
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to DUSA_PSEPK: tRNA-dihydrouridine(20/20a) synthase (dusA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K05539, tRNA-dihydrouridine synthase A [EC: 1.-.-.-] (inferred from 77% identity to dia:Dtpsy_1883)MetaCyc: 60% identical to tRNA-dihydrouridine synthase A (Escherichia coli K-12 substr. MG1655)
1.1.1.-
Predicted SEED Role
"tRNA dihydrouridine synthase A"
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Carotenoid biosynthesis - General
- Insect hormone biosynthesis
- Nucleotide sugars metabolism
- Porphyrin and chlorophyll metabolism
- Puromycin biosynthesis
- Trinitrotoluene degradation
- alpha-Linolenic acid metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.-.-.-
Use Curated BLAST to search for 1.-.-.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (317 amino acids)
>GFF4791 tRNA dihydrouridine synthase A (Hydrogenophaga sp. GW460-11-11-14-LB1) MMDWTDRHCRYLHRLLSKHTLLYTEMVTTGALTHGSVQRHLRFNAEEHPVALQLGGSDPA DLAAAARLGEQWGYDEINLNCGCPSDRVQRGAFGACLMAEPQTVADGVKAMVDAVSIPVT VKHRIGIDRIESYDFVRDFVGTVSEAGCSVFLVHARNAWLDGLSPKENREIPPLRYELAH RLKQDFPQLTFGINGGFTTNAQIADQLREVDGVMVGREAYHNPWLLTSWDEAFFGAAPST ITREAVEEQMVAYMERAFLEDGCPWYAIARHMLGLRHGLRGARRWRQIWSDHRLKPLPPR EVWAMAQAHVGLEAEAA