Protein Info for GFF4783 in Variovorax sp. SCN45

Annotation: Penicillin acylase (EC 3.5.1.11)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 830 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF01804: Penicil_amidase" amino acids 50 to 818 (769 residues), 705.9 bits, see alignment E=4.6e-216

Best Hits

KEGG orthology group: K01434, penicillin amidase [EC: 3.5.1.11] (inferred from 88% identity to vpe:Varpa_3993)

Predicted SEED Role

"Penicillin acylase (Penicillin amidase) (EC 3.5.1.11)" (EC 3.5.1.11)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.11

Use Curated BLAST to search for 3.5.1.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (830 amino acids)

>GFF4783 Penicillin acylase (EC 3.5.1.11) (Variovorax sp. SCN45)
MKRMNLGAVALAVLALAGCASSPSGSGGPSGGSTPTRPSSSFAIPGLEKPAEVLVDRWGV
PHLYAGTLYDAFVAQGFIAARDRLWQMDLWRKRGLGEMAKDFGPAWVESDRAARAVLYRG
DMYREWLAYGSDAKRVAEAFTAGVNAYVAQVRAQPALLPTEFALLGYQPATWSPEDVVRI
RHHGLTLNFTSEIERARAFCAGGVGIKADWLRRELDPPVTPRVPQGLDPCGIPATELRAA
YLRATEPPQFTKANTRLGLNAGAQVPVALLPGSGSSTDTAQSGDPTGAYGSNNWVISPKL
TATGRPILANDPHRSHGAPSLRYMTHLSAPGMDAIGAGEPFLPGLSIGHNGTIAFGLTRF
YMDQEDLYVYQLNPRNPAEYRYQGRWEPMVRVTERIAVKGESTPREVVNTFTRHGPVLLA
EPGKQRAYALRAAWLEPGMAPYFGSMDYMRARNWDQFRAAMNRWGAPGENQVYADTSGNV
GWIPGGLTPIRPNWDGLMPVPGDGRYEWSGFRNGDELPSEFNPARGYVVTANENNIPPDH
PAAKKGVGYEWSDAARARRLKELFAAKVAAGSRFTVEDSERMQNDIVATPAQRLLKLLAG
LRSDDAQTAAALRMLQGWDGSMDRDSGAAALYEVWSSKSLRSAVLKAGAGDAGAVLATPG
DNTRMVLLLENPSGWVTNEQRDALLLKTLPLAMQELSAKLGPDTAAWKWGTLHRAEFRHP
LGGVVDAATRKKLDVGDWPMSGSAFTPMAATYRPSDYKLTAGASFRMVLDVGNWDASRVI
NTPGQSGNPDSPNYRDLAPLWLEGKYVPLVYTRAAVEKETVERIQLTPGK