Protein Info for GFF4776 in Variovorax sp. SCN45

Annotation: Broad-specificity amino acid ABC transporter, substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF13458: Peripla_BP_6" amino acids 26 to 361 (336 residues), 357.7 bits, see alignment E=1.5e-110 PF01094: ANF_receptor" amino acids 48 to 321 (274 residues), 31.9 bits, see alignment E=1.2e-11 PF13433: Peripla_BP_5" amino acids 161 to 338 (178 residues), 40.1 bits, see alignment E=3.9e-14

Best Hits

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 93% identity to vpe:Varpa_3999)

Predicted SEED Role

"Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (397 amino acids)

>GFF4776 Broad-specificity amino acid ABC transporter, substrate-binding protein (Variovorax sp. SCN45)
MKKTAVFSAVSLAIALAAGAAQAQEKVKIGFITDMSSLYADVEGKNGALAIQMAIDDFGG
KVNGMPIELLQADHQNKADIAASKAREWIDTQGLTMLFGGTSSGTGLAMAKVAQEKKRVF
IVNGAGSSALTNEQCSPYTVHYAYDTVALAKGTGSAVIARGDKSWFFLTADYAFGQALEA
DASKVVKEKGGTVVGSVKHPLNTSDFSSFLLQAQNSKAQVLALANAGGDTQNAIKAAKEF
GISKTMKMVGLLVFVTDVHSLGLKNTEGLLLTTSWDWNLDDKTRAFGKRFFEKTKRMPTD
IQAADYSATMTYLKAVQAAKSIDADTVMAQIKKTPIDDFYAKGFVREDGRFVHDMYLVQV
KSPAESKQPWDYYKVVQKLKGEEVFTTKAESKCALWK