Protein Info for GFF4734 in Variovorax sp. SCN45

Annotation: Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 822 PF07992: Pyr_redox_2" amino acids 10 to 308 (299 residues), 193.1 bits, see alignment E=2.6e-60 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 11 to 800 (790 residues), 927.6 bits, see alignment E=3.8e-283 PF00070: Pyr_redox" amino acids 152 to 234 (83 residues), 74.7 bits, see alignment E=2.4e-24 PF18267: Rubredoxin_C" amino acids 325 to 391 (67 residues), 64.6 bits, see alignment E=2.4e-21 PF04324: Fer2_BFD" amino acids 429 to 476 (48 residues), 50.9 bits, see alignment 5.5e-17 amino acids 493 to 543 (51 residues), 50.5 bits, see alignment 7.2e-17 PF03460: NIR_SIR_ferr" amino acids 568 to 629 (62 residues), 58.2 bits, see alignment 1.9e-19 PF01077: NIR_SIR" amino acids 638 to 775 (138 residues), 108.1 bits, see alignment E=1e-34

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 84% identity to lch:Lcho_2678)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (822 amino acids)

>GFF4734 Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4) (Variovorax sp. SCN45)
MDMRVKTRQKLVMVGNGMAGVRTIEELLKVEPDLYDITVFGAEPHPNYNRILLSPVLAGE
QTVDEIILNPWSWYEENNITLHAGKKVASVDRVKRIVRAEDGTEAAYDRLLLCTGSNPFM
LPVPGAKLEGVIAYRDIADTNLMIETAKTHKHAVVIGGGLLGLEAANGLMKRGMQVTVVH
VMPWLMERQLDEVAGKLLQKSLEDRGMKFLMGAQTQELIGSEDGRVKAIRFKDGLEVPTD
LVVMAVGIRPNTALAESMRLHVNRGIVVTDTMQTVTDARIYSVGECAAHRGIAYGLVAPL
FEQAKVAANHLAMFGIGRYMGSLTSTKLKVTGIDLFSAGDFQGGEGTEEIVMSDPFGGVY
KKLVIKDDKLVGACLYGDTVDGSWYFKLLRDGRSVHDIRDKLMFGESNIGDTGHEGHNKA
ASMPDDAEVCGCNGVNKGTICKAIKEKGLFTLDEVKKHTKASASCGSCTGLVEQILMFTA
GGDYSATPKKKAVCGCTDVSHQDVRDAIRKQHYLTHDEVYRNLGWRTPNGCATCRPAINY
YLISTWPKEAKDDPQSRFINERSHANIQKDGTYSVIPRMWGGHTTPDELRRIADAADKYK
IPTVKVTGGQRIDLLGVKKEDLENVWKDIGMPSGFAYAKSLRTVKTCVGSEWCRFGTQDS
TQMGKDLERALWAMYSPHKVKLAVSGCPRNCAEAGIKDVGVIGVDSGWELYVGGNGGIKT
EVAQFLVKVKTAEEVMEYSGAFLQLYREEGWYLERTVHYIGRVGLDYVKKKILDDAEGRK
ALWERLQFALDGEPDPWFESAQASVDVRQFTPLAVPQVEKTA